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DIGITAL-BIO TECHNOLOGY

Automatic Mammalian Cell Counter

See how this cell counter achieves high precision and incredible ease of use in a video here:

ADAM Cell Counter Design Video

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Product Information


Features

New standard of automatic cell counting
  • Measures total cell numbers and cell viabilities through cutting-edge detection technologies. Instead of tryphan blue staining which can lead an inaccurate data, ADAM utilizes sensitive fluorescence dye staining, LED optics and CCD detection technologies to make the cell analysis more accurate and reliable
  • Combined with a disposable microchip, the operation of Automatic Cell Counter is now extremely simple, easy and cost-effective
  • The need for routine calibration of the device is completely eliminated
  • Cleaning and warm-up time is no longer necessary with ADAM
  • The disposable chip greatly reduces the risk of the exposure to hazardous samples, such as patients’ blood samples, virus infected cells, etc
Accurate Cell Counting
  • ADAM automatic cell counter is a precision microscope integrated with fluorescence optic and image analysis software
  • After the samples are stained with fluorescent dye, propium iodide, which intercalates DNA to stain the nucleus of target cells, fluorescent images are taken automatically
  • The system takes 22 images of each of the 4 channels by the movement of automatic X-Y stage
  • The obtained images are processed by image analysis software integrated inside the system
  • All procedures are automatic once the stained sample has been dropped to the disposable chip
Disposable Microchips
  • ADAM automatic cell counter utilizes a precision disposable microchip to eliminate the problem of a permanent counting chamber
  • Each microchip has 4 different channels, each can be either use for total cell count or viability count
  • Each disposable microchip is produced by state-of-the-art manufacturing facility and validated for accuracy

Fig. 1. Correlation of total cell counting between hemocytometer and ADAM using CHO cells

Fig. 2. Samples with low, medium and high concentrations of cells were counted with ADAM

Fig 3. Comparison of the cell viabilities between ADAM and flow cytometry. SK-OV, HeLa and NCI-H23 cells were treated with 100, 100uM H2O2 for 3 hours, then analyzed by ADAM and flow cytometry. Values are given as means +/- SD of three experiments

Easy Operation

Images

ADAM PC Software (optional to control the instrument)

Images

Images

The Graph section of the software shows information of cell size distribution and counting results of each frame that ADAM captured.

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Specifications

ADAM-MC
Objective Lens4X
Excitation source532nm green LEDs
CCD cameraB/W CCD
FilterEmission filter
Measuring range50 000- 4 millions cells/ml
Analysis timeless than 1 minute
Loading sample volume12ul (minimum)/channel (half is staining, half is cell media) for 4-channels chip; 20ul (minimum)/channel for 2-channels chip
Measuring volume5.4ul/channel for 4-channels chip; 9 ul/ channel for 2-channels chip
Weight9 kg
Size (WxLxH)220x375x250mm
Accessories (included)Key pad, external video monitor, optional PC control software, one kit AD4K-200 (for up to 800 cell counts)

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Ordering

DescriptionCatalog no.Price
Automatic Cell Counter: Main device + Starter kit + Keypad + LCD monitor + Starter kit AD4K-200 with 200 4-channel chips and staining + Optional PC SoftwareADAM-MCTBD
Consumables Available
DescriptionCatalog no.Price
AccuChip Kit: 200 pcs AccuChip, kit with stain solution (Max, 800 tests/kit)AD4K-200TBD
AccuStain: Stain solution (1 000 tests/kit)ADR-1000TBD

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Millenia Systems Martin Guralnik
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