Frequently Asked Questions (FAQ)You will find here the answers to a series of questions that have been asked to us on numerous occasions. Use the list below to select the topic that interests you. Topics
What is the difference between RPM and RCF in regard to tubes?RPM stands for revolutions per minute. This is how centrigue manufacturers generally describe how fast the centrifuge is going. The rotor, regardless of its size, is revolving at that rate. The force applied to the contents varies with the size of the centrifuge rotor. RCF, or relative centrifugal force is measured in force × gravity, or g-force. This is the force exerted on the contents of the rotor, resulting from the revolutions of the rotor. It is RCF, not RPM, that separates aqueous solutions in the centrifuge. RCF can be calculated for any centrifuge with the following equation: RCF = 1.1118 × 105 × r × RPM2 Where r is the radius of the rotor in cm. Back to Top
What is the Corbett Life Science Rotor-Gene™?The Rotor-Gene™ is a multiplex realtime DNA amplification system. The fluorescent energy of each sample tube can be monitored in realtime during a thermal cycling profile. Back to Top What reaction volume is suitable for use in the Rotor-Gene™?The thermal cycling conditions of the Rotor-Gene™ are optimized for 20…25μl reaction volumes. Depending on the application, as little as 5μl to 10μl can be run to minimize reagent costs. Back to Top How can pipetting errors be minimized?The more volume is used for pipetting, the more accurate the results will be. This is the same for hand pipetting or pipetting with a robotic system. Errors are minimized by increasing the volume. Back to Top |