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Mithras LB 943 Monochromator Multimode Reader

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Mithras LB 943
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Mithras LB 943 Monochromator Multimode Reader

Enhanced Versatility and Well-known Reliability

Mithras² LB 943 is a high-end microplate multimode reader based on monochromator technology with excellent performance. Renowned for its sensitivity and robustness especially in luminescence and BRET measurements the reader supports all important reading technologies including:

  • Luminescence
  • BRET and BRET²
  • Fluorescence
  • FRET
  • Fluorescence Polarisation (FP)
  • UV/VIS Absorbance
  • AlphaScreen® and AlphaLISA®
  • Time-Resolved Fluorescence
  • TR-FRET

In contrast to the usual kind of multi-technology instruments the Mithras multimode microplate reader has been designed with a proprietary optical system consisting of separate paths for different reading technologies (mDOPS), thus offering performances known from dedicated instruments. As each technology has its own demands on the optical system only separated light paths ensure that the needs for high sensitivity and a wide dynamic range are met with each technology.

 

Features

Double Monochromators

Mithras² LB 943 employs two double monochromators for excitation and emission respectively. The monochromators are predominately meant to provide entire wavelength flexibility in absorbance and fluorescence measurents, yet they can be used for Time-Resolved Fluorescence and Luminescence measurements as well. While a low f number and a high blocking ensure exceptional performance variable bandwidths and increments of 1 nm illustrate the versatility of the monochromators.

 

Built-in Reagent Injectors

Up to 4 reagent injectors can be set up to dispense multiple activators or detection reagents at any time and with user-adjustable volumes during the measurement. Berthold Technologies´ injectors are based on the proprietary JET Injection technology. Superior accuracy and precision is combined with excellent mixing performance. Variable injections speeds and "friendly" material enable even injection of live cells (e.g. in Aequorin based GPCR screening). The injector tips can be located right in reading position enabling the measurement of very fast labels and reactions, e.g. Acridinium esters and Calcium flux, and to inject activators into a running kinetic.

 

Waste Pump

Excess fluids from priming and washing procedures are safely discarded via an automatic waste pump.

 

Temperature Control

A temperature controlled incubation area ensures stable conditions for the microplate whenever temperature sensitive enzymes or cells are in use. Condensation of lidded microplates is reduced.

 

Automatic Plate Height Adjustment (Z Optimization)

The plate height adjustment module detects the height of the plate used and automatically adjusts the measurement optics for it. There is no need any more for users to perform time-consuming Z-scans. Besides optimising the focus of the excitation beam it also ensures there will be no crosstalk from adjacent wells.

 

More Information
Applications (optional modules may be requiered)

The Mithras² LB 943 has been developped as consistent successor of the Mithras LB 940, thus all applications conducted with the latter can be performed with the Mithras² as well. Improvements in optics and the availability of monochromators even increase its usefulness for said applications.

  • Reporter Gene
  • Second Messenger
  • BRET and BRET²
  • Cytotoxicity
  • ATP determination
  • Calcium monitoring
  • AlphaScreen®/ ADLOC
  • Caspase assay
  • Kinase assay
  • Protease assay
  • Cellular luminescence / Phagocytosis
  • Immunoassays
  • Chemiluminescence Immunoassay
  • Chemiluminescence Immunoassay with Acridinium Ester
  • SNP determination
  • Apoptosis
  • Enzyme kinetics
  • Proteasome and Cathepsin B activity
  • Cell density / Cell proliferation
  • Cell density assay with crystal violet
  • Nucleic acid quantification
  • DNA quantification
  • Protein quantification
  • Protein quantification with Coomassie Brilliant Blue
  • Binding Assay

Further Applications

  • Chemotaxis
  • ChromaGlo™ assays
  • Cytokine analysis
  • DNA probe assays
  • Ion channel studies
  • GPCRs / 7TM receptors
  • Membrane potential
  • Proteases
  • Protein concentration
  • Protein-protein interactions
Detection Devices 2 low-noise photomultiplier tube operated in single photon counting mode, spectral range up to 850 nm ultra-low-noise photomultiplier tube operated in single photon counting mode, spectral range up to 650 nm (HiSens position) photo diode, spectral range 2
Monochromator f number: 2.7 (high light transmission) variable bandwidth: 6 - 22 nm increment: 1 nm blocking: 10-6
Applications Luminescence BRET, BRET² Fluorescence (top) FRET Absorbance
Injectors (Maximum) 3 injectors Volume: 10 – 100 μL JET injection technology Accuracy better 2 % Precision better 2 %
Shaking 3 modes, 3 velocities
Scanning area up to 10,000 points per well
Kinetics Operation minimum resolution 0.05 sec maximum duration up to 7 days
Automation robot integration
Luminescence <2 amol ATP per well <2 zmol Firefly luciferase per well (equals less than 800 molecules)
Fluorescence < 0.2 fmol FITC per well (filter) <2 fmol FITC per well (monochromator)
Crosstalk 5 x 10-6
Dynamic range (linearity) >6 decades (PMT) / 4 decades (photo diode)
Temperature Control PID Controlled microprocessor touch pad, Digital Display
Interface USB 2.0
OS Win XP, Win Vista, Win 7
PC Requirements Pentium Processor, 500 MHz (or better), CD ROM drive, display 1024 x 768 (or better), USB
Regulations CE, UL, CSA
Net Weight 48 kg
Light Source halogen lamp Xenon flash lamp laser, 680 nm (Alpha Technology option)
Microplate Formats 6 – 1536 well plates Terasaki plates Petri dishes filter membranes
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