Spot Hunter-35 00 300
Herolab
This robotic system integrates a fluorescence detection unit for automatic extraction of selected protein spots after 2-D electrophoresis The Spot Hunter is the new generation of proteomic workstations offering utmost flexibility, accurateness and reliability on minimal footprint.
Features
The combination of the three essential steps of proteomic analysis – image capture, selection and extraction – in one housing provides a faster and more efficient work.
How it works
- The image is captured from 2D gels stained with all common methods like Coomassie Blue, Silver Stain or fluorescent dyes like SYPRO® Ruby with an application-specific configured high-resolution CCD camera. All Types are equipped with a White Light LED Transilluminator.
- The easy4science software detects interesting protein spots, creates a target list and transfers it to the picking unit.
- The selected spots will be automatically extracted under contamination free conditions and then transported into one – or with the High Throughput Type into four – standard microtiter plates (96 or 384 wells).
Other Features
The light-tight housing enables the detection of fluorescent dyes. The gel supporting surface is illuminated and can be optionally cooled in order to avoid deformation of gels. Control and positioning of the x- and y-axes of the picking unit is carried out with the help of two mini cameras which make the process visible on the computer monitor (available as an optional extra).
A unique feature of the Spot Hunter is the simultaneous and speed-conform suction of the spot during the downward movement of the needle. Depending on the gel quality, the cylindrical form of the punched-out spot will not be damaged. The software easy4science is supplied with a high performance Quad Intel computer system including Windows® XP Professional and 24” TFT-Monitor (Windows® 7 on request). The GLP conform documentation of the process enables an exact backtracking of the spot. For 96 picks the Spot Hunter needs no longer than 20 minutes.
| Working speed | Extraction of 96 spots in less than 20 minutes |
|---|---|
| max gel size | 340 x 290 mm |
| Picking area | 300 x 250 mm |
| Gel Plate | also works as Calibration Plate for adjustment of camera optics and picking unit |
| Camera | Monochrome scientific grade CCD came Real time and integration time |
| Connection | for inert gas supply into the robot’s interior |
| Computer | High-end Windows® PC with high-resolution monitor for control of the entire process |
| Corpus | Aluminum corpus with leveling feature and computer controlled motors of all axes, including the intelligent picking device with easy to change syringe and needle |
| Housing | Light-tight housing for detection of fluorescent dyes with computer controlled CCD camera |
| Software | Eco System Software supports all chemistries and a variety of applications, including absolute quantification, relative quantification, allelic discrimination, and high resolution melt curve analysis (HRM) |
| maximum height with camera hood | 115 cm (45 inches) |
| Dimensions | 51 x 37 x 23 cm (20 x 14.6 x 9.1 inches) |