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Accuchip 4x Kit (max 400 tests) with Accustain Solution

Catalog Number:
AD4K-200
Availability: In stock
$845.00

Accuchip 4x Kit (max 400 tests) with Accustain Solution

Adam is based on staining mammalian cell DNA with a fluorescent dye, Propidium Iodide (PI). PI does not enter cells with intact cell membranes or active metabolism. In contrast, cells with damaged membranes or with inactive metabolism are unable to prevent PI entering the cell. As a result, the nuclei of non-viable cells will only be stained. AccuStain Solution T for the total cell counting is composed of the fluorescent dye (PI) and lysis solution.

AccuStain Solution N for the non-viable cell counting is composed of the fluorescent dye and PBS. In order to measure the total concentration of cells, the plasma membranes of all the cells must be disrupted to stain all the Nuclei with PI. The process of disrupting and staining is achieved by treatment with AccuStain Solution T. In the second solution, live cells remain intact and are not stained. Only the non-viable cells are stained and detected.

 

   Optimal range

  •    5ⅹ104 ~ 4ⅹ107 cell/mL
  •    5ⅹ104 ~ 4ⅹ106 cell/mL

   Loading sample Vol.

  •   20 µL ( for Accuchip 2x )
  •   12 µL ( for Accuchip 4x )

   Measuring sample vol.

  •    8.5 µL ( for Accuchip 2x )
  •    3 µL ( for Accuchip 4x )
More Information
Material PMMA(polymethly methacrylate)
Outer size 25 mm(W) x 75 mm(H)
Grid System Neubauer Improved grid
Chamber depth 0.1 mm(now available); 0.05 mm/0.02 mm(available features)
Chamber volume 10ul (now available); 2 ul (available features)
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