MATra-S Immobilizer, 200 ul (for up to 7 million cells)

BA

Suspension cells have to be made adherent first by incubating them with the reagent MATra-S Immobilizer which contains magnetic nanoparticles (MagTag™). Then MATra-A Reagent (or MA Lipofection Enhancer with IBAfect) loaded with the nucleic acid can be applied and MATra (or Magnet Assisted Lipofection) can be performed.

Protocol

• 1. Dilute the cells to be transfected to 5 x 10^5 - 1 x 10^6 per ml in medium. The medium may contain serum or supplement or may be serum-free; depending on cell type and sensitivity of cells towards serum-free conditions.
• 2. Mix cell suspension with 30 µl of well vortexed MATra-S Immobilizer per 1ml of cell suspension.
• 3. Incubate for 10 - 15 min.
• 4. Distribute 100 µl of the cell suspension per well of a flat-bottom 96-well plate placed on the 96 Magnet Plate. Use the surface factor of the Table under section 3.1 for other formats. Universal Magnet Plates are recommended for all but 96 well plates.
• 5. Incubate for 15 min.
• 6. During the incubation continue with the standard protocol for MATra-A. Do not perform the medium change of step 3 of the MATra-A protocol. Keep cells on the Magnet Plate until the 15 minute incubation of step 6 of the MATra-A protocol has been terminated.