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NightSHADE LB 985 Plant Imaging System

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NightSHADE
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NightSHADE LB 985 Plant Imaging System

Berthold Technologies has developed the new NightSHADE imaging system with plant research in mind.


Applications

For applications such as gene research, circadian rhythms, regulation of plant growth, stress tolerance and drug screening the NightSHADE will offer you unique features. Low level Bioluminescence from luciferases as well as Chemiluminescence e.g. from luminol enhanced reactive oxygen species can be measured. Of course Fluorescence from GFP and other fluorescence dyes can be monitored in the NighSHADE, too.

 

Sample format

You can image small seedlings in Petri dishes or microplates, leaves, fungi, up to whole plants.

 

Cameras

You can select between three different instrument models: As entry level NightSHADE LULu is equipped with an sensitive emCCD camera for bio- and chemiluminescence measurements. For higher demands on luminescence sensitivity the NighSHADE IKLu is equipped with an supersensitive low noise CCD camera. The instrument of choice for both, luminescence and fluorescence measurements, is the NighSHADE LUFlu. The instrument - additionally to the emCCD camera - is equipped with an fluorescence unit including illumination and one GFP filter set.

 

Cabinet

The absolutely light-tight dark chamber is equipped with dual flange for multiport access. As standard the camera is mounted on the top and can be optionally mounted at side by using the camera conversion kit. The instrument can be equipped with two cameras, one on top and one at side, too.

 

Software

The indiGOTM software allows complete hardware control. Extensive tools for image evaluation such as zoom function, peak search, overlay, colour scale and surface plot are implemented. You can collect single pictures as well as image sequences. All images can be exported to TIF files. Raw data and processed data are filed separately according to GLP rules.

 

Accessories

Various accessories are available:

  • LED Plant Growth Illumination
  • X/Y Table
  • Turn Table
  • Turn table for square Petri dishes
  • Temperature control
  • Humidity control
  • Gooseneck spot illumination
  • Ringlight epi illumination

 

Technical Details

Camera type Back-lit midband-coated full frame chip, 1024 x 1024 pixel, slow scan mode
Pixel size 13 x 13 µm
Spectral range 350-1050 nm
Quantum efficiency 90% at 620 nm
Pixel binning Variable to increase sensitivity, up to 16x16
Exposure times From ms to hours
CCD cooling Thermoelectrical air cooling down to -70° C
Size

25 mm, f 0.95

C-mount for most efficient light collection
Field of view

Standard lens: 270 x 270 mm

Macro lens: 130 x 130 mm

Lamp type 75 W halogen lamp
Spectral range 340-750 nm
Inner dimensions 520 x 400 x 360 (W x H x D)
Connections Flange for light tight port, mounting areas for side view mounting option and LED panels, software-controlled mains socket.
Power Supply 110-240 V, 50/60 Hz; max 400 VA; minimum 4 free sockets
Temperature range Max 30° C
Humidity 10-80%, non-condensing
Bench Load capacity of at least 45 kg (weight of instrument); minimum size 900 x 600 mm (W x D) plus space for PC
Software IndiGO™ software for instrument control and data analysis
Interface USB 3.0
Regulations CE, EN
Dimensions (W x D x H) 600 x 400 x 920 mm
Weight 45 kg
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