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MBI EVOlution Probe qPCR mix (No Rox) 4ml (1000 reactions)

Catalog Number:
MBI-P1000
Availability: In stock
$825.00

For in vitro use only

Description:

MBI EVOlution 5 x Probe qPCR Mix (no ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and MBI Hotstart DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

MBI Hotstart DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

 

Applications:

  • Detection and quantification of DNA and cDNA targets
  • Profiling gene expression
  • Microbial detection
  • Viral load determination

Mix Composition:

  • MBI Hotstart DNA Polymerase
  • 5 x Probe qPCR buffer
  • 15 mM MgCl2

1 x PCR solution – 3 mM MgCl2

  • dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP
  • No ROX dye

 

Recommended qPCR reaction mix:

Component

Volume

Final conc.

MBI EVOlution 5 x Probe qPCR Mix (no ROX)

4 µl

1x

Primer Forward

(10 pmol/µl)

0.4-0.8 µl

200-400 nM

Primer Reverse

(10 pmol/µl)

0.4-0.8 µl

200-400 nM

Probe

1 µl

100-250 nM

DNA template

1-5 µl

1-50 ng/µl

H2O PCR grade

up to 20 µl

 

Total

20 µl

 

 

Recommended qPCR cycles:

Cycle step

Temp.

Time

Cycles

Initial denaturation

95ºC

15 min

1

Denaturation

95ºC

15-20 s

40

Annealing/Elongation

60ºC

60 s

 

IMPORTANT: To activate the polymerase, include an incubation step at 95ºC for 15 minutes at the beginning of the qPCR cycle.

Safety warnings and precautions:

This product and its components should be handled only by persons trained in laboratory techniques. It is advisable to wear suitable protective clothing, such as laboratory overalls, gloves and safety glasses. Care should be taken to avoid contact with skin or eyes. In case of contact with skin or eyes, wash immediately with water.

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.

Shipping and Storage conditions:

Routine storage: -20ºC

Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of MBI EVOlution 5 x Probe qPCR Mix (no ROX).

More Information
Amount of Reactions 1000 Reactions
Mix Composition No ROX Dye
Application qPCR Probe
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