My Cart

MATra-A reagent 200 ul (for 200 ug nucleic acids)

Catalog Number:
7-2001-020
Availability: In stock
Please contact us

IBA

Magnet assisted Transfection (MATra) is a new, easy-to-handle and highly efficient method to transfect cells in culture. Using this new technique nucleic acids, such as plasmid DNA, oligonucleotides or siRNA, are in a first step associated with magnetic particles. Exploiting magnetic force the full nucleic acid dose is then rapidly drawn towards and delivered into the target cells leading to efficient transfection. For MATra, cells must adhere to the bottom of the culture vessels which makes special precautions necessary for suspension cells.

For indicated cells the following methods were tested. 1: Calcium phosphate vs Magnet Assisted Lipofection, 2-4 Lipofection vs Magnet Assisted Lipofection, 5: Lipofection vs Magnet Assisted Transfection. Data kindly provided by industrial IBA customer.

 

Magnet Assisted Transfection (MATra) with MagTag

Magnet Assisted Transfection (MATra) is a new, easy-to-handle, very fast and highly efficient technology to transfect cells in culture. All types of nucleic acids from plasmid DNA or siRNA to oligonucleotides can be used with the MATra approach. Data from variety of species using cell lines or primary tissue culture have accumulated including human, monkey, mouse, rat, xenopus, pig, cat or fish.

Using this new technique, nucleic acids are in a first step associated with specific magnetic nanoparticules (MagTag). Exploiting magnetic force the full nucleic acid dose is then drawn towards and delivered into the target cells leading to efficient transfection without disturbing the membrane architecture, without causing chromosomal damage or leaving a hole in the cell membrane like other transfection technologies. Cellular uptake occurs by either endocytosis or pinocytosis. Delivered to the target cells, the DNA is released into the cytoplasm. The magnetic particles are accumulated in endosomes and / or vacuoles. Over time, the nanoparticules are degraded and the iron enters the normal iron metabolism not influencing cellular functions.

Two approaches are possible: for a standard Magnet Assisted Transfection "MATra-A Reagent" is used; for more critical cells it is also possible to combine the MATra technology with lipofection ("Magnet Assisted Lipofection"). For this purpose, we are offering "MA Lipofection Enhancer" and the high-efficiency lipofection reagent "IBAfect". The MA Lipofection Enhancer can also increase the efficiency of viral transfections.

Both techniques can be used with the adherent cells as well as with suspension cells. However, for the latter cells have to be localized at the bottom of the cell culture plate using the MATra-S Immobilizer. MATra can also be adapted to high-throughput transfection assays using robotic stations and adapted protocols.

For indicated cells the following methods were tested. 1: Calcium phosphate vs Magnet Assisted Lipofection, 2-4 Lipofection vs Magnet Assisted Lipofection, 5: Lipofection vs Magnet Assisted Transfection. Data kindly provided by industrial IBA customer.

 

Ten reasons to use MATra

  • Greatly improved transfection rates
  • Very gentle method with almost no toxicity; membranes remain intact
  • No transfection related side effects on your model system and read out
  • Only low vector doses are required
  • Easy protocol; transfections can be performed in original culture plates
  • Functional with serum and serum-free medium
  • Successfully used on many and also critical cell lines (almost 200 cell types tested positively so far)
  • Highly cost-effective; no need for expensive instruments (like electroporator or particle gun)
  • Combination with lipofection or viral transfection possible
  • Works excellently for various types of nucleic acids including siRNA or antisense oligo transfections

 

Tips

MATra is applicable at different phases of cell confluence, however in general we recommend 50-70% confluence (in some systems a higher visual confluence may result in higher Magnet Assisted Transfection rates).

MATra is non-toxic at recommended amounts in most cell systems. Still, if higher DNA/MATra or Enhancer amounts are used a medium change is advised within 2 hours.

Write Your Own Review
You're reviewing:MATra-A reagent 200 ul (for 200 ug nucleic acids)
Brochure (661.92 kB)