Confocal detection
- Optimize fluorescence detection
- Assure good focalization all along the scan
- Less background
- SNR is increased by 2.5 times
Microarray Scanner - MBI Innoscan 1100
Numéro de catalogue:
MBIIS1100
Disponibilité:
En stock
Microarray Scanner - MBI Innoscan 1100
Multiplexed whole-slide imaging
- 0.5μm pixel size, equivalent to 20x microscope magnification
- High sensitivity : high performance analogical PMTs
- 3-color simultaneous confocal detection
Easy automation
- Real-time autofocus or in-content focus system
- 24-slide autoloader for high-throughput applications
- Automated image acquisition with Mapix software
Reliable and traceable results
- Barcode reader
- Accurate control of acquired images
- Scanner supplied with a validation slide which verifies scanner performance
Technologie
Real-time autofocus
- Detection idone through a spatial phinhole
- Follows the slide movement and deformation in real time during the scan
- Avoid all out-of-focus signal
Automatic barcode reader
- Reliable and traceable results
All standard microscope slides format
- Glass
- Nitrocelluose coated glass
- Plastic
- Silicon wafer
- Gold coated glass
Dynamic range
- From > 104 to >106
- Detect the lowest signals avoiding saturation.
Technical specifications
| PIXEL SIZE | 0.5 - 40μm | ||
| LASER EXCITATION WAVELENGTH | 488nm | 532nm | 635nm |
| COMPATIBLE FLUOROPHORES* | Cy2, FITC Alexa Fluor 458, Alexa Fluor 488 |
Cy3 Alexa Fluor 544, Alexa Fluor 555 |
Cy5, DRAQ 5 Alexa Fluor 647 |
| LASER POWER | Adjustable (2 laser powers) | ||
| LOADER CAPACITY | 1 slide | ||
| DETECTION TYPE | Real-time confocal with 3 analog photomultipliers (PMT) | ||
| PMT GAIN | Adjustable from 0 to 100% (Linear from 1 to 100%) |
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| OPTICAL FILTER | 7 position filter wheel** 1 standard fluorescence and 1 neutral density filter per channel;5 additional filters on request |
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| FOCUS | Real-time autofocus Manual focus: offset adjustment 300 μm range, 1 μm increment |
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| SLIDE SIZE | Compatible with all standard microscope slides: 25-26 x 75-76 mm2 / 1” x 3” ; thickness: 0.9 - 1.2 mm | ||
| SCANNING AREA | Adjustable up to 22 x 74 mm2 | ||
| SCANNING SPEED | From 10 to 35 lines/second (l/s) 3.6 minutes per slide at 10 μm and 10.5min for an area 22x22 mm2 at 1μm |
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| DYNAMIC RANGE | > 104 in normal mode or > 106 in dynamic extension mode | ||
| UNIFORMITY | > 95% | ||
| BARCODE READER | Automatic barcode reading | ||
| INTERFACE | Ethernet interface | ||
| IMAGE FORMAT | TIFF (16-bit and 20-bit in dynamic extension mode) | ||
| POWER SUPPLY | ~ 100-240 VAC, 1.2 A, 50-60 Hz | ||
| ACQUISITION SOFTWARE | MAPIX (image acquisition and spot quantification software) | ||
| DIMENSIONS (LXDXH) | 316 x 549 x 432 mm3 12.5” x 21.6” x 17.0” | ||
| WEIGHT | 30 kg (66.1 lbs) | ||
| Résolution | from 0.5 to 40 µm/pixel |
|---|---|
| Longueur d'onde d'excitation | 488nm, 532nm and 635nm |
| Vitesse de scan | De 20 à 35 lignes par seconde (25 lignes par seconde maximum pour une taille de pixel de 0,5 µm). 3,5 minutes pour numériser une lame entière à 10 µm/pixel et 3 couleurs. 10,5 minutes pour une zone de 22 x 22 mm² à 1 µm/pixel et 3 couleurs. |
| Surface de scan | 22 x 74 mm^2 |
| Plage dynamique (Linéarité) | 10^6 |
| Laser | Diode laser, réglable (2 puissances laser disponibles) |
| Gain PMT | Linéaire de 0 à 100 % (par pas de 0,1 de 0,1 à 1 %, et de 1 de 1 à 100 %). |
| Autofocus | Automatique ou manuel |
| Detection | Analyse confocale à photomultiplicateur analogique (PMT) |
| Format des puces | Compatible avec toutes les lames de microscope standard : 25-26 x 75-76 mm² / 1" x 3" ; épaisseur : 0,9 - 1,2 mm |
| Interface | ethernet |
| Format d'image | TIFF 16 bits et TIFF 20 bits |
| Lames d'échantillon | Toutes lames de microscope standard de 25 x 75 mm ^ 2/1 x 3 " |
| Lecteur de code-barre | Oui |
| Poids net | 30 kg |
| Optionnel : Logiciel du cahrgeur automatique | Intégration totale dans le logiciel MAPIX venir avec le Innoscan Scanners |
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