EVE™ HT FL — High-Throughput Fluorescence Cell Counter

The EVE™ HT FL is a high-throughput fluorescence cell counter engineered for biopharmaceutical process development and cell therapy manufacturing QC. Combining brightfield imaging with dual-fluorescence channels (AO/DAPI), it processes 48 samples in a single run using a disposable 48-channel plate compatible with multi-channel pipettes. The system delivers accurate total cell counts, viability percentages, and cell size distributions for cell lines, primary cells, stem cells, and PBMCs — with minimal sample consumption and optional 21 CFR Part 11 compliance for regulated environments.

Core Features

High-Throughput 48-Sample Processing

The disposable 48-channel counting plate is designed for multi-channel pipette compatibility, enabling efficient loading and mixing without the delays of manual hemocytometer counting. The entire 48-sample run completes in under 4 minutes, making the EVE™ HT FL ideal for:

• Cell line screening and media optimization campaigns
• Bioprocess development and scale-up monitoring
• QC release testing in cell therapy manufacturing
• High-frequency viability monitoring across multiple conditions

Complex Sample Accuracy — PBMCs, Stem Cells, CAR-T

Traditional brightfield counting struggles with contaminated or complex samples containing red blood cells, platelets, and cellular debris. The EVE™ HT FL's dual-fluorescence approach identifies nucleated cells only, delivering accurate counts where Trypan Blue methods fail. This makes it particularly effective for:

• PBMC preparations from whole blood or apheresis products
• Stem cell and iPSC culture monitoring
• CAR-T cell manufacturing and potency assessment
• Primary cell cultures with high debris content

Performance Validation

Software & Data Management

The intuitive software interface displays total cell count, live/dead distribution, viability percentage, and cell size histograms in real time. Export capabilities include:

• One-click PDF test reports for regulatory submissions
• CSV raw data export for downstream analysis
• JPG image export for presentations and documentation
• Real-time bar graphs and scatter plots during acquisition
• Windows 10 / Windows 11 compatible

21 CFR Part 11 / GMP Compliance (Optional)

The optional compliance module safeguards all records and data in accordance with FDA requirements for electronic records and signatures. Features include:

• User management and role-based access control
• Electronic records and electronic signatures
• Complete audit trail logging every action with date, time, and user details
• Data modification restricted to authorized users only

Technical Specifications

Applications

• High-throughput cell line screening and bioprocess development
• PBMC counting and viability assessment in blood-derived products
• Stem cell and iPSC culture monitoring and expansion QC
• CAR-T cell manufacturing, potency assessment, and release testing
• Primary cell analysis in high-debris or RBC-contaminated samples
• Regulated GMP manufacturing with optional 21 CFR Part 11 compliance
• Media optimization and cell growth curve studies

EVE™ HT FL counting plates and staining reagents sold separately. Specifications subject to change without notice.