ExTransfection Electroporation system

ExTransfection - Electroporation system 

Introducing Microporation Technology

NanoEntek developed microporation technology to overcome the limitations of traditional electroporation methods using cuvettes. We are proud to introduce the ExTransfection™, an innovative pipette-type electroporation system that dramatically improves transfection efficiency.

The core of microporation technology lies in its use of a capillary-type electric chamber, replacing the conventional cuvette. This breakthrough maximizes the gap size between electrodes while minimizing the electrode surface area, significantly enhancing both transfection efficiency and cell viability. The increased electrode gap ensures a more uniform electric field within the capillary, further improving transfection performance.

Advantages of the ExTransfection™ Electroporation System

The ExTransfection™ is a cutting-edge, benchtop electroporation device featuring a single electroporation chamber within its pipette tip. With precise electrical pulses, it efficiently delivers nucleic acids, proteins, and siRNA into all mammalian cell types while maintaining high cell viability.

Applications : Transfection efficiency

High Transfection Efficiency for Cell Lines

The transfection efficiency of microporation has always been reliable. Various cell lines have been extensively tested with GFP gene transfection, and their efficiencies were analyzed using fluorescence microscopy and flow cytometry.

High Transfection Efficiency for Primary Cells

Primary cells are widely recognized as the ideal material for animal cell research due to their physiological similarity to live animals. However, the transfection of genetic material into primary cells has long been a significant challenge. The ExTransfection™ consistently delivers successful transfection results across various primary cells

Specifications

• Reaction volume: 10 μL or 100 μL
• Cell concentrations: 5 × 10 4 - 5 × 105 cells for 10 μL tip, 5 × 10 5 - 5 × 10 6
  Pulse voltage: 500 - 2,500 V
• Pulse width 1 - 100 ms
• Number of pulses 1 - 10
• User interface 8 inch touch screen
• Electrical rating: 100 - 240 VAC, 4.0 A, 50/60 H
  Electrical input: 24 V, 7.5 A, 180 W
• Weight: 6 Kg
• Device dimensions: 243.5 (W) x 352.3 (D) x 231.6 (H) mm

Ordering information Cat. No