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Orion II LB 965 Microplate Luminometer (Discontinued)

Numéro de catalogue:
84031
Disponibilité: En rupture de stock
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***This product has been discontinued by the manufacturer***. However, we are pleased to offer you the following model which has been designed to replace it: Centro LB 963 Microplate Luminometer

 

 

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Orion II Microplate Luminometer

High flexibility Plate Luminometer

Orion II is a state-of-the-art instrument offering complete configuration flexibility. It features high sensitivity thanks to highly efficient optics and photon counting mode. Reagents can be connected easily, and the low dead volume (only 450 µL) saves costly reagents. Orion II can be equipped with up to four reagent injectors and temperature control up to 50°C. Double Injection Design (DID) allows parallel processing of samples and saves valuable assay time

 

HIGHLIGHTS

High performance luminescence

The outstanding sensitivity of Orion II is the result of the unique design, advanced engineering, and high-quality workmanship. All functions of the Orion II are seamlessly integrated to render superior, user-friendly performance.

The detector is operated in photon counting mode, which guarantees the lowest signal background for excellent signal to noise ratio and the highest linearity. The plate adjustment mechanism automatically compensates for variations in microplate size. The built-in safety circuit protects the detector from potential damage resulting from accidental exposure to high levels of light. In addition, a warning is produced should a sample ever exceed measurement range.

 

Powerful crosstalk control – reliable results

Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples?  Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The special design of the Orion II does almost eliminate this problem: with a crosstalk of 3 x 10-5, you never have to worry about the position of your high-intensity samples in your plate.

 

Up to 4 injectors with low dead volume and flexible positioning

The combination of reagent addition options is virtually unlimited. Up to four automatic reagent injectors are available with injection volumes of 10 to 150 μL each. For increased throughput, up to 2 injectors can be installed in measurement position for simultaneous injection. The optimized reagent holders accommodate any shape and any size bottle in a position that allows the complete use of valuable reagent.

 

Optimized injection for faster throughput

The Orion II system has the capability to run samples and process measurements in the most time efficient manner. The proprietary Double Injection Design (DID) allows parallel processing of samples saving valuable assay time. DID has been applied to both 96 well and 384 well configurations.

 

TECHNICAL DETAILS

Measurement

Detectors

Low-noise photomultiplier tube in single photon counting mode

Detection technologies

Luminescence

Measurement modes Endpoint, Kinetics, Repeated, Batch
Spectral range luminescence 300-600 nm

 

Injectors

Injectors Up to 4 syringe injectors
Injector volume 10-150 µL in 1 µL steps

 

Microplates

Microplate formats

96- and 384-well

 

Environmental Control

Shaking Linear, orbital, cross shaking, with selectable speed
Temperature control From ambient +5° to 42° C or to 50° C (optional)

 

Performance

Luminescence sensitivity <10 amol ATP/well (96-well plate)
Crosstalk 3 x 10-5
Dynamic range 6 orders of magnitude

 

Operation

Interface Windows software, connected to computer by USB
Operating system Windows 7 or better
Available software packages  
  • Simplicity for Raw Data included by default
  • Additional licenses available for Dual Assay, Fast Kinetics and Batch Protocol

 

Additional Specifications

Power Supply 110-240 V, 50/60 Hz, 30 VA (maximum 70 VA with 4 injectors and heating)
Temperature tolerance Storage: 0-40° C
Operation: 15-30° C

Humidity tolerance

10-80% non-condensing

Dimensions (W x D x H)

385 x 410 x 255 mm

Weight

22 kg

Plus d’information
Format de l'échantillon Plaque microtitration de 96 puits, opaque, sous forme solide ou en bande, avec configuration disponible pour les plaques de 96 puits et de 384 puits.
Detecteur Tube photomultiplicateur avec cathode au bialkali, plage de sensibilité spectrale efficace de 300 à 600 nm, utilisé en mode de comptage de photons.
Sensibilité Under 1.0 amol ATP
Diaphonie Less than 3 x 10-5.
Plage dynamique (Linéarité) 6 decades.
Temps de mesure 0.1–100 s per well.
Plaque de 96 puits Oui
Microplaques de 384 puits Oui
Injecteurs (Maximum) Jusqu'à quatre par système Orion II (extensible). Deux injecteurs en pré-position, deux en position de mesure.
Volume d'injection 10–150 µl par pompe avec des incréments de 1µl.
Format d'injection Conception à double injection (DID) : Injection simultanée dans deux puits adjacents possible en mode colonne ainsi qu'en mode rangée.
Tuyauterie Chemically inert PTFE tubing and connections (PTFE; KEL-F; glass; PS), easily exchangeable liquid handling system and tips. Minimal dead volume due to short reagent lines.
Protocole d'amorçage Amorçage avant et arrière, contenant d'amorçage externe ou contenant d'amorçage intégré (en option).
Automatisation Compatible avec tous les dispositifs de transfert de microplaques connus
Interfaces Interface série (RS-232) et interface USB pour PC.
Dimensions W 385 mm, D 410 mm, H 255 mm.
Agitation Secouage linéaire, orbital et croisé.
Poids net 22 kg.
Puissance 230 V 50 Hz; 115 V 60 Hz.
Puissance [W] 30 VA, maximum 70 VA (4 injectors and heating).
Conditions de stockage 0– 40°C.
Plage de température de fonctionnement 10– 30°C
Plage de longueur d'onde 300 – 600nm
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