Tristar 5 Multimode Reader
Tristar 5 Multimode ReaderApplication flexibility you can count onThe Tristar 5 is a modular high-performance microplate reader equipped with independent, user-selectable filters and monochromators on both, the excitation and emission side for any measurement. This guarantees both, flexibility and sensitivity whenever you need it. The system supports advanced detection modes such as TRF and TR-FRET, and FP as well as specific assays like BRET/BRET2, NanoBRET™, Lantha-Screen® or AlphaScreen®. Detection TechnologiesThe Tristar 5 Multimode Microplate Reader provides you with application flexibility for today, tomorrow, and beyond to master your changing plate reading applications. The system supports the following wide range of detection modes:
FlexTec Optics – when flexibility AND sensitivity countThe Tristar 5 system is equipped with FlexTec Optics, offering you the best of two worlds – benefit from the flexibility to easily select a discrete wavelength and perform spectral scans for both, excitation and emission using its built-in monochromator technology. Or optimize your assays´ sensitivity by utilizing the system filter sliders for excitation and emission. Or mix both technologies if required: the Tristar 5 delivers both, flexibility and sensitivity in a single system. The Tristar 5 employs up to two double monochromators providing blocking properties needed in sensitive fluorescence assays. Both monochromators are equipped with software driven continuous bandwidth variation to optimise the instrument for the specific demands of different assay conditions. Berthold´s flexible monochromator technology offers you variable bandwidths from 4-12 nm in excitation and 8 – 22 nm for emission, selectable in 1 nm increments. ONE-4-ALL Optics - uncompromised performanceOur patented ONE-4-ALL optics have been optimised to combine the stability and user-friendliness of a multimodal optical system with the sensitivity and versatility of dedicated optical devices. ONE-4-ALL optics work for both filter- and monochromator-based applications, so there´s never a compromise in performance in any mode. High-performance filter system - when sensitivity countsThe quick-change filter technology of the Tristar series provides you with the flexibility required to meet your application needs: up to 40 different excitation and emission filters can be easily mounted on exchangeable filter slides. The filters are characterized by high transmissions properties which can be up to 25-fold that of monochromators. Technologies like Time-Resolved Fluorescence (TRF) can be measured more efficiently with filters. Furthermore, filters with a large bandwidth are available to analyze fluorophores with wide spectra and all luminescence-based assay requiring to use filters, e.g. BRET, BRET2 or NanoBRET™ assays. Ergonomic design - supporting your workflowsThe ergonomic design of the system provides full front access for all key operations (e.g. plate loading, filter change and connecting reagents). Reagent vials can be stored in an integrated front compartment, providing easy access and visibility. It contains a removable trough that can be filled with water or ice to keep all reagents cooled. Different reagent tube holders are available to ensure secure handling of valuable reagents. The system has a flat surface on its top, providing enough space to put down a laptop. Powerful crosstalk control – reliable resultsDo you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples? Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Tristar 5 does almost eliminate this problem: with a crosstalk of 10-6, you never have to worry about the position of your high-intensity samples in your plate. JET injectors: highest accuracy, speed and cell-friendlinessThe Tristar 5 can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence. Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.
Convenient Software optionsChoose between different software options according to your application needs: ICE Software
MikroWin Software
Technical Details
|
| Sélection de la longueur d'onde | 2 double monochromateurs |
|---|---|
| Technologies de mesure | Luminescence BRET, BRET² Fluorescence (top) FRET Absorbance UV & VIS Time-Resolved Fluorescence TR-FRET / HTRF® FP (Fluorescence Polarization) AlphaScreen® |
| Luminescence | 6 amol/well ATP (plaques de 96 puits) |
| Fluorescence | < 7 amol par puits de FITC (384 puits) |
| Gamme d'absorbance | Précision supérieure à 2% Précision supérieure à 0,6% |
| Seuil de détection de fluorescence par unité de temps | Moins de 5 amol par puits. |
| Plage dynamique (Linéarité) | 6 ordres de grandeur (compteur de photons) 0-3,5 OD (photodiode) |
| Diaphonie | 5 x 10-6 |
| Unité d'injection | up to 3 injectors, JET injection technology variable volumes: 10 – 100 μL speed 200 – 440 μL/sec accuracy better 2% (over entire range of volume) precision better 2% (over entire range of volume) injections into microplates with up to 384 wells |
| Agitation | 3 modes, amplitude et vitesse variables. |
| Contrôle de la température | +5°C au-dessus de la température ambiante jusqu'à 45°C. |
| Formats des microplaques | De 6 à 384 puits, solides et en bande, dimensions de 128 x 86 mm (L x l), hauteur de 14,0 à 21,0 mm (adaptateurs nécessaires). Convient aux boîtes de Petri de 35 et 60 mm, aux plaques Eppendorf μPlate G 0,5 et aux cuvettes standards (avec capuchon). |
| Interface | USB |
| Système d'exploitation PC | Windows 7 (32/64 bit), Windows 10 (32/64 bit) |
| Type de PC requis | Processeur de type Pentium (2 GHz ou mieux / Intel Core iX recommandé), 1 port USB disponible. |
| Certifications | CE, NRTL |
| Source de courant | 100 – 240 VCA ±10% 50 / 60 Hz Classe I |
| Voltage | 24 VDC ± 5% |
| Puissance [W] | 140 VA |
| Dimensions | 391 x 470 x 395 mm |
| Detecteur | Tube photomultiplicateur à faible bruit en mode double, plage spectrale de 280 à 650 nm (jusqu'à 850 nm avec PMT à plage étendue). Photodiode : plage spectrale de 200 à 1000 nm. |
| Source lumineuse | Lampe flash au xénon |
| Plage de longueur d'onde | 200 - 1000nm |
