Vilver Fusion Absolute - The First Imaging System With Absolute Protein Quantification

Vilber Fusion Absolute

The First Imaging System With Absolute Protein Quantification

Absolute imaging, limitless research. The Vilber Fusion Absolute is the first ultra-precise photon-counting imaging system that delivers effortless absolute quantification through NIST-Traceable Calibration. By measuring light irradiance in photons/s/pixel and referencing certified standards, it ensures unmatched accuracy, reproducibility, and cross-condition comparability. Unlike relative methods that only report fold-changes between samples, absolute quantification provides precise, reliable values in absolute units, transforming your workflows with standardized protocols and directly comparable data.

Calibrated Photon Counting Optics

The Fusion Absolute quantifies the incident light on each protein band detected on a membrane, providing an estimate of the light power received per unit area (photons/s/cm²). Measuring irradiance overcomes the need to adjust binning, lens aperture, or exposure time, since it directly reflects the amount of light reaching the membrane surface. AI-defined regions of interest (ROIs) identify each band, and calibration curves based on NIST-traceable standards convert detected light into an absolute quantity of protein.

Absolute NIST Traceable Calibration Imaging is based on traceable references provided by the National Institute of Standards and Technology (NIST).

Exceptional Performance

Trusted by Nobel Prize winners and prestigious institutes worldwide, the Fusion range owes its reputation to state-of-the-art optics. The Fusion Absolute combines a custom f/0.70 aperture lens (the smaller the f-number, the better) with a deeply cooled CCD scientific camera for the best possible images without background noise. Its wide linear dynamic range captures both strong and weak signals in a single exposure, ensuring accurate quantification of low-concentration proteins.

·        Widest f/0.70 aperture: maximum sensitivity at picogram level

·        Absolute cooling up to -65°C (FX7): via multi-stage Peltier

·        Scientific grade 16-bit CCD camera: capturing 65,535 grey levels

·        Wide linear dynamic range: 4.8 OD

Multispectral Imaging And Multiplexing

While traditional chemiluminescent Western blots target one protein at a time, the system's epifluorescence capabilities enable multispectral imaging, so you can target and visualize two or more proteins within a single protocol. Nine Absolute Spectra Capsules (365, 440, 480, 540, 580, 640, 680, 740, and 780 nm) sit precisely on the spectrum of your dye to avoid crosstalk, and each module is equipped with premium emission filters exhibiting a flat wavelength peak for enhanced illumination uniformity. A powerful N.M.F. (non-negative matrix factorization) unmixing algorithm solves crosstalk and autofluorescence issues for more accurate detection in multi-labeled samples.

Simplified Imaging Process And AI-Powered Analysis

·        Wizard button: the system automatically recognizes your samples and suggests the correct protocol for faster acquisition

·        Advanced motorized optics: the Z-axis moves automatically according to the nature and size of the sample

·        Virtual Blot technology: an augmented representation of your blot displays all quantification information at a glance

·        AI-assisted analysis: automatically generates a comprehensive dashboard of quantitative data

·        License-free software with unlimited users

·        Data export: one-click to Excel, 16-bit TIFF format for image storage

Applications

·        Western, Northern, and Southern blotting and protein gels

·        DNA and RNA gels

·        Colorimetric-stained gels, X-ray film, SSCP gels, colony dishes, and flasks

·        Genomics (DNA mutation, methylation, RNA gene expression) and proteomics (protein molecular weight, mutation, expression)

Two Configurations To Choose From

Feature	Fusion Absolute FX6	Fusion Absolute FX7
Positioning	Everyday imaging excellence	Ultimate sensitivity for advanced research
Design	PC-Based	Integrated PC with 15.6" touchscreen
Dimensions / weight	678 x 507 x 382 mm | 50 kg	682 x 528 x 382 mm | 59 kg
Camera	Scientific grade 16-bit CCD, 65,535 grey levels	Scientific grade 16-bit CCD, 65,535 grey levels
Resolution	Up to 20 MP (2823 x 2224 native)	Up to 10 MP (2160 x 2160 native)
Cooling	Up to -30°C absolute (three-stage Peltier)	Up to -65°C absolute (four-stage Peltier)
Lens aperture	Fixed f/0.7	Fixed f/0.7
Dynamic range	4.8 OD	4.8 OD
Field of view	Up to 24 x 20 cm	Up to 20 x 20 cm
Binning modes	1x1, 2x2, 4x4, 8x8	1x1, 2x2, 4x4, 8x8
Imaging modalities	Chemiluminescence, fluorescence and epifluorescence	Chemiluminescence, fluorescence and epifluorescence
Transillumination	Interchangeable pads (UV-A, UV-B, UV-A+UV-B, white, blue, green)	Interchangeable pads (UV-A, UV-B, UV-A+UV-B, white, blue, green)
Multispectral imaging	Up to 9 Absolute Spectra Capsules (365 to 780 nm)	Up to 9 Absolute Spectra Capsules (365 to 780 nm)

Compatible Fluorophores And Reagents

·        Fluorophores: Alexa 488, Cy2, Alexa 532, Alexa 555, Alexa 680, Alexa 700, Alexa 750, Cy7, Alexa 790

·        Intercalating agents: Ethidium Bromide, SYBR Safe, Midori Green, Gel Red, SYBR Green

Absolute Design

The Fusion Absolute is a robust all-in-one standalone system made of aluminium and stainless steel, resistant to the most demanding decontamination procedures. The stainless steel darkroom features a wide-access door (110° angle), UV safety, and easy handling of the interchangeable transillumination pads. Choose the PC-based version or the integrated 15.6" touchscreen, the widest on the market, with adjustable screen orientation. Connectivity: Ethernet and Wi-Fi, 3 USB 3.0, 1 USB 2.0, 1 HDMI.