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Accuchip 4x Kit (max 400 tests) with Accustain Solution
Availability:
In stock
$845.00
Estimated shipping date 06/05/2024, order this item in the next
Accuchip 4x Kit (max 400 tests) with Accustain Solution
Adam is based on staining mammalian cell DNA with a fluorescent dye, Propidium Iodide (PI). PI does not enter cells with intact cell membranes or active metabolism. In contrast, cells with damaged membranes or with inactive metabolism are unable to prevent PI entering the cell. As a result, the nuclei of non-viable cells will only be stained. AccuStain Solution T for the total cell counting is composed of the fluorescent dye (PI) and lysis solution.
AccuStain Solution N for the non-viable cell counting is composed of the fluorescent dye and PBS. In order to measure the total concentration of cells, the plasma membranes of all the cells must be disrupted to stain all the Nuclei with PI. The process of disrupting and staining is achieved by treatment with AccuStain Solution T. In the second solution, live cells remain intact and are not stained. Only the non-viable cells are stained and detected.
Optimal range
5ⅹ104 ~ 4ⅹ107 cell/mL
5ⅹ104 ~ 4ⅹ106 cell/mL
Loading sample Vol.
20 µL ( for Accuchip 2x )
12 µL ( for Accuchip 4x )
Measuring sample vol.
8.5 µL ( for Accuchip 2x )
3 µL ( for Accuchip 4x )
More Information
Material
PMMA(polymethly methacrylate)
Outer size
25 mm(W) x 75 mm(H)
Grid
System Neubauer Improved grid
Chamber depth
0.1 mm(now available); 0.05 mm/0.02 mm(available features)
Chamber volume
10ul (now available); 2 ul (available features)
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