FlowStar² LB 514 Radio Flow Detector for HPLC

FlowStar² LB 514 Radio Flow Detector for HPLC

The FlowStar² represents the latest improvements in an almost 50 years history of radio flow monitors used in liquid chromatography. An optimised detector and electronics design for ultimate sensitivity has been combined with touch-screen technology for quick and easy operation. In addition the FlowStar² can be operated via the RadioStar radio HPLC software or via the HPLC system´s software.

Features

High Sensitivity

Low noise photomultipliers operated with an optimized coincidence circuitry and luminescence subtraction provide the best sensitivity available.

Automatic Cell Detection

A chip in the flow cell´s mounting frame contains all necessary cell information and ensures the correct cell has been inserted for the measurement to be performed.

Isotope Library

The instrument relies on an extensive library with preset settings for the energy windows of e.g. ¹⁴C, ³H, ³²P, ³³P, ³⁵S, ¹²⁵I, ¹¹¹In and ^99mTc. The integrated scanning feature improves the detector setup for the isotopes.

Both, single and dual isotope labelled samples can be measured.

User Access Control

User access can be controlled by applying a security password to protect the instrument from unauthorized use and interfering with running measurements.

Control via PC or via touch screen

Any mode of operation can be applied

• touch screen
• RadioStar radio HPLC software
• HPLC system software

Multiple Interfaces

With its analog outputs and digital interfaces, the FlowStar can be connected to any HPLC system.

Quality Control

An automatic performance control can be set up in the instrument´s software taking care of a regular and automatically started QC performance check.

Accessories

Liquid Scintillant Pump

The pump is separated from the detector eliminating any potential damages caused by leakages. The pump is entirely controlled by the radio detector or the PC software respectively.

The double piston design provides pulsation-free operation and a wide range of the flowrate between 0.001 and 10 mL per minute matches all applications and flow rates of the main HPLC system.

Static Mixer

Two cartridges with 50 and 150 µL volumes are available for efficient mixing of eluent and scintillant.

Splitters

To preserve part of the eluate for further investigations splitters direct only part of it to the scintillant mixer and detector

Waste Valve

As only part of the eluate is radioactive a waste valve can be applied to divert only the radioactive fraction into a specific container.

RadioStar

Besides controlling the detector, the PC software offers sophisticated functions for radio chromatographic data evaluation

• recording of 4 channels: 2 isotopic and 2 non-isotopic signals
• integration of peaks
• half-life correction
• quench correction
• report generation
• export functionality, e.g ANDI format

The software is 21 CFR part 11 compliant.

Applications

Metabolism studies

H-3 & C-14 Improved liquid chromatography by VHPLC: Cuyckens et al. (2008).Improved liquid chromatography—Online radioactivity detection for metabolite profiling.Journal of Chromatography 1209 (1–2), 128–135 

C-14 Nicotinamide Release: Berger et al. (2007). Regulation of poly(ADP-ribose) polymerase 1 activity by the phosphorylation state of the nuclear NAD biosynthetic enzyme NMN adenylyl transferase 1. PNAS 104 (10), 3765-3770 

Development of radiochemicals

I-123 Zmuda et al. (2015): Synthesis and Evaluation of a Radioiodinated Tracer with Specifity for Poly(ADP-ribose) Polymerase-1 (PARP-1) in Vivo. J. Med. Chem. 58(21), 8683-8693 

Sephton et al. (2012): Synthesis, radiolabelling and in vitro and in vivo evaluation of a novel fluorinated ABP688 derivative for the PET imaging of metabotropic glutamate receptor subtype 5. American Journal of Nuclear Medicine and Molecular Imaging 2(1), 14-28 

Pesticide monitoring

C-14 activity of treated soil: Jablonowski et al. (2012). Long-term persistence of various 14C-labeled pesticides in soils. Environmental Pollution 168, 29–36 

PET

F-18 Herth et al. (2009): Radioactive Labeling of Defined HPMA-Based Polymeric Structures Using [18F]FETos for In Vivo Imaging by Positron Emission Tomography.Biomacromolecules 10 (7), 1697–1703 

C-11 Mu et al. (2014): Synthesis and Preliminary Evaluation of a 2-Oxoquinoline Carboxylic Acid Derivative for PET Imaging the Cannabinoid Type 2 Receptor.Pharmaceuticals (Basel)  7(3): 339–352 

Plant Research

Analysis of C-14-Labeled Pigments: Beisel et al. (2010). Continuous Turnover of Carotenes and Chlorophyll a in Mature Leaves of Arabidopsis Revealed by 14CO2 Pulse-Chase Labeling. Plant Physiology 152 (4), 2188-2199 

Environmental Research

C-14 Testosterone and Estradiol in soil: Jacobsen et al. (2005). Persistence of Testosterone and 17ß-Estradiol in Soils Receiving Swine Manure or Municipal Biosolids;Journal of Environmental Quality 34, 861-871