BCL6/IGH gene fusion t(3;14) detection probe (BCL6/IGH)

BCL6/IGH Gene Fusion t(3;14) Probe Detection Kit - 100µL/10 Tests

The BCL6 gene located on 3q27 is translocated in 40% of diffuse large B-cell lymphomas. Reciprocal translocations can involve a number of other chromosomal locations, including 14q32 (IGH), 2p11 (IGK), and so on, all juxtaposing to 3q27. BCL6 is thought to mediate the DNA-binding activity of a number of zinc-finger transcription factors and characterizes the germinal center B-cell gene signature in microarray studies. The presence of the BCL6 translocation in DLBCL carries a favorable prognosis, in comparison with those carrying BCL2 who have a worse outcome and those with neither translocation who have an intermediate prognosis.

Product Main Components

The kit consists of BCL6/IGH dual-color probes

Intended use

The reagent carries out in situ hybridization staining on the basis of routine staining to provide doctors with auxiliary information for diagnosis. The test results are only for clinical reference and should not be used as the only basis for clinical diagnosis. Clinicians should comprehensively judge the test results in combination with the patient's condition, drug indications, treatment response and other laboratory test indicators.

Detection principle

Fluorescence in situ hybridization is a technique for directly observing specific nucleic acids in cells in vitro. According to the principle of base complementary pairing, the specific probe is complementary to the target sequence in the cell. Due to the fluorescence of the probe, the gene state of the hybrid probe and the target sequence can be clearly observed under the fluorescence microscope under the appropriate excitation light.

Applicable Instruments

Fluorescence microscopy imaging systems, including fluorescence microscopy and filter sets suitable for DAPI (367/452), Green (495/517), and Orange (547/565).