BCL6 ; MYC ; IGH ; BCL2/IGH gene detection probe (IGH/BCL2)

BCL2/IGH t(14;18) Gene Fusion Probe Detection Kit - 100µL/10 Tests

IGH (Immunoglobulin Heavy Locus) is a Protein Coding gene. Diseases associated with IGH include Agammaglobulinemia 1, Autosomal Recessive and Follicular Lymphoma 1. Among its related pathways are Immune response CD16 signaling in NK cells and Immune response Lectin induced complement pathway.

BCL2 (BCL2 Apoptosis Regulator) is a Protein Coding gene. Diseases associated with BCL2 include Follicular Lymphoma 1 and High-Grade B-Cell Lymphoma Double-Hit/Triple-Hit. Among its related pathways are Apoptosis and survival-Anti-apoptotic action of nuclear ESR1 and ESR2 and Estrogen signaling pathway. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and identical protein binding. 

The majority of patients with follicular lymphoma and a subset of patients with diffuse large B-cell lymphoma (DLBCL) have a translocation between chromosomes 14 and 18 which results in the fusion of the immunoglobulin heavy chain gene (IGH), located at chromosome region 14q32, with the BCL2 gene, located at chromosome region 18q21.

Product Main Components

The kit consists of BCL2/IGH dual color probe

Intended use

The reagent carries out in situ hybridization staining on the basis of routine staining to provide doctors with auxiliary information for diagnosis. The test results are only for clinical reference and should not be used as the only basis for clinical diagnosis. Clinicians should comprehensively judge the test results in combination with the patient's condition, drug indications, treatment response and other laboratory test indicators.

Detection principle

Fluorescence in situ hybridization is a technique for directly observing specific nucleic acids in cells in vitro. According to the principle of base complementary pairing, the specific probe is complementary to the target sequence in the cell. Due to the fluorescence of the probe, the gene state of the hybrid probe and the target sequence can be clearly observed under the fluorescence microscope under the appropriate excitation light.

Applicable Instruments

Fluorescence microscopy imaging systems, including fluorescence microscopy and filter sets suitable for DAPI (367/452), Green (495/517), and Orange (547/565).