DEK/NUP214 gene fusion detection probe (DEK/NUP214)
DEK/NUP214 Gene Fusion Probe Detection Kit - 100µL/10 Tests
DEK (DEK Proto-Oncogene) gene encodes a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases.
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP214 (Nucleoporin 214) gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome.
Product Introduction
The kit uses orange fluorescein-labeled NUP214 probe and green fluorescein-labeled DEK probe to bind DEK/NUP214 probe to the target detection site by in situ hybridization.
Product Main Components
The kit consists of DEK/NUP214 color probe
|
Component name |
Specifications |
Quantity |
Main components |
|
DEK/NUP214 dual color probe |
100μL/Tube |
1 |
NUP214 Orange probe, DEK Green probe |
Applicable Instruments
1. Fluorescence microscopy imaging system includes fluorescence microscope and filter sets. The kit is labeled with orange fluorescein,
and the filter set compatible with the fluorescent labeled dye should be selected.
- DAPI: The maximum excitation wavelength is 367nm and the maximum emission wavelength is 452nm.
- Orange fluorescence: The maximum excitation wavelength is 547nm and the maximum emission wavelength is 565nm.
- Green fluorescence: The maximum excitation wavelength is 495nm and the maximum emission wavelength is 517nm.