FB12 Luminometer (Discontinued)

***Discontinued***

Please see equivalent: Sirius 2 LB 9526 Tube Luminometer

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Berthold - FB12 Luminometer

The FB12 system is the ideal choice for stable glow luminescence of single samples from a variety of sample formats. FB12 Luminometer combines a very small footprint with excellent sensitivity and outstanding ease of use.

Concept

In stand-alone mode FB12 is ready for standard measurements. Samples are readily loaded on a pull out-drawer. Closing the sample drawer automatically initiates the measurement without pressing any key. An external printer may be connected optionally.

The modular Windows® Luminometer PC-Software or the Pocket PC- Luminometer Software can further enhance performance by organizing the measurement dialog and data evaluation.

Detector

The FB12 Luminometer features advanced photoncounting electronics coupled with a highly sensitive detector, providing the lowest signal background and highest linearity spanning 6 decades. No cumbersome internal calibration is required and no gain switch is needed.

Light collection is maximized through both the proximity of the sample to the detector and the use of optimized light reflectors surrounding the sample vial. As a result, reporter gene assays using firefly luciferase can now detect less than 1000 molecules of the enzyme with the FB12 Luminometer.

Stand-Alone Operation

FB12 operates either under its own microprocessor software or fully integrated under PC operation. In stand-alone mode FB12 supports basic raw data measurement and printer output. Three softkeys allow easy and intuitive operation. Both delay and measurement time can be set in steps of 0.1s.

Windows® PC Software

FB12/Sirius PC Software is designed for stationary use in the laboratory. In addition to the Quick Measurement function, it offers five additional protocol types: Single Assay, Dual Assay, Single Kinetics, Multiple Kinetics and Cut-off Assay. The operator can define new protocols of all five types. Highly flexible graphical presentation of kinetic data is supported.

Pocket PC Luminometer Software

Pocket PC Luminometer software runs on a pocket PC connected by a serial cable to ensure trouble- free operation even in a difficult environment. Replicate and Cut-off protocols.

Applications

FB12 is the instrument of choice for all luminescent glow type applications performed in single sample format, for example:

• Reporter gene assays, including dual-reporter assays
• ATP assays
• Cell proliferation, cytotoxicity and biomass assays
• Apoptosis assays
• Detection of mycoplasma contaminations in cell cultures
• Cellular luminescence / reactive oxygen species
• Environmental toxicity and mutagenicity assays

The FB12 Luminometer has been evaluated by the Research Department staff at Promega Corporation and has been certified for use with their Dual Luciferase® "DLReady" (trademark of Promega Corp.) assay.