LUNA Reusable Slide (Discontinued)
***This product was discontined and we do not offered anymore.***
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Cell counting is an essential step in routine cell maintenance and for obtaining accurate and consistent experimental results. In recent years. automated cell counters have become more popular because affordable instruments have become increasingly available at competitive prices. However, many researchers still use the hemocytometer to avoid the expenses associated with the use of disposable slides. To overcome this problem, Logos Biosystems designed a reusable slide (LUNA" Reusable Slide. L12008) to be used with the LUNA" family of automated cell counters. Here, we compare the cell counting results of the LUNA'" Reusable Slide, a hemocytometer, and disposable slides.
MATERIALS AND METHODS
Comparing cell counting accuracy of the LUNA Reusable Slide and a hemocytometer
To determine the accuracy of cell counts done with the LUNA" Reusable Slide, cell counts were compared to those obtained manually with a hemocytometer. Cells were serially diluted and mixed with an equal volume of 0.4% trypan blue stain. 10µL of the mixture was loaded into the LUNA Reusable Slide and a glass hemocytometer with Neubauer counting grids (Marienfeld). To obtain cell concentrations with the glass hemocytometer, the hemocytometer was imaged with the iRiS" Digital Cell Imaging System and the cells within the four comer squares of the Neubauer counting grids were counted. Cell samples in the LUNA" Reusable Slide were counted with the LUNA-II" Automated Cell Counter. All experiments were performed in triplicate.
Evaluating cell counting accuracy of the LUNA Reusable Slide with brightfield and fluorescence aumated cell counters Thetoperformance of the LUNA Reusable Slide for brightfield and fluorescence cell counting was evaluated by using two stains, nypan blue and acridine orange/propidium iodide (AO/PI), respectively. For brightfield counting, cells mixed 1:1 with trypan blue were loaded into the LUNA" Reusable Slide or disposable slides and counted with the LUNA", LUNA-FL'", and LUNA-II" automated cell counters. For fluorescence cell counting, 2 AL AO/PI was mixed with 18µL cell suspension and 10µL was loaded into the LUNA" Reusable Slide or disposable slides and counted with the LUNA-FL" Automated Fluorescence Cell Counter. All experiments were performed in triplicate.