MBI Evolution UV-Vis Microplate Reader Monochromator base (200-1000nm)
MBI Evolution UV-Vis Microplate Reader
200-1000nm Monochromator-Based System with Incubation & Shaking
The MBI Evolution UV-Vis Microplate Reader is a high-performance, monochromator-based laboratory instrument designed for precise absorbance measurements across the full UV-Vis spectrum. With a wavelength range of 200 to 1000 nm and 1 nm step resolution, this versatile microplate reader is ideal for life science research, pharmaceutical development, and clinical diagnostic laboratories across Canada. The system supports both 96-well and 384-well microplates and features integrated incubation and shaking functions, making it a complete solution for ELISA, protein quantification, nucleic acid analysis, and kinetic assay applications.
Key Advantage: Unlike filter-based plate readers, the MBI Evolution uses a grating monochromator with a dedicated reference channel, delivering superior wavelength accuracy and long-term stability without the ongoing cost of replacement filters.
Applications
The MBI Evolution UV-Vis microplate reader supports a comprehensive range of absorbance-based assays commonly performed in modern laboratories:
ELISA & Immunoassays
Perform quantitative enzyme-linked immunosorbent assays with high sensitivity and reproducibility across 96-well and 384-well formats.
Protein Quantification
Compatible with BCA, Bradford, and Lowry assays. Measure protein concentration accurately with linearity up to R²≥0.999.
Nucleic Acid Detection
Quantify DNA and RNA samples using UV-Vis absorbance at 260 nm and 280 nm for purity assessment.
Enzyme Kinetics
Monitor reaction rates in real time with kinetic detection mode, integrated shaking, and precise temperature control.
Cell Viability & Cytotoxicity
Run MTT, XTT, and resazurin assays with incubation up to 45°C for mammalian and bacterial cell studies.
Spectral Scanning
Capture full absorbance spectra of unknown samples from 200-1000 nm to identify peaks and characterize compounds.
Why Choose the MBI Evolution Microplate Reader?
Double-Beam Optical System with Reference Channel
The MBI Evolution employs a double-beam optical design featuring dual silicon photoelectric detectors—one for sample measurement and one for real-time reference correction. This architecture automatically compensates for light-source fluctuations, ensuring exceptional baseline stability and data reliability across extended kinetic runs and high-throughput screening campaigns.
Monochromator vs. Filter-Based Detection
As a monochromator microplate reader, the MBI Evolution eliminates the limitations of fixed-wavelength filter systems. Researchers can select any wavelength from 200 to 1000 nm in 1 nm increments without purchasing or installing additional filters. This flexibility reduces long-term operating costs and enables full spectral scanning capabilities that filter-based microplate readers cannot provide.
Long-Life Xenon Flash Lamp
The system is powered by a high-energy xenon flash lamp rated for 10⁹ flashes, delivering consistent illumination across the entire UV-Vis range. Automatic calibration of the light source, grating, detector, and positioning system occurs at startup, ensuring that every measurement meets rigorous accuracy standards.
Integrated Incubation & Shaking
Temperature-sensitive assays benefit from the built-in incubator, which maintains conditions from RT+4°C to 45°C. Linear shaking with three adjustable speed options ensures homogeneous mixing of reagents and uniform cell culture conditions—critical for reproducible ELISA and cell-based assay results.
Intuitive Android Touchscreen Interface
The 10-inch capacitive touchscreen runs on Android and supports external keyboard and mouse connectivity. The onboard software handles endpoint, kinetic, and spectral scanning protocols without requiring an external computer, though the system is also fully compatible with MBI ReaderIt-II PC analysis software for advanced data processing and export.
Software & Data Management
User Authority & Security
The integrated software supports multi-level user management with password-protected accounts. Administrators can create sub-user profiles, restrict access to specific functions, and maintain full audit trails. A guest account enables quick access for routine measurements without compromising experiment confidentiality.
Analysis Tools
- Blank subtraction and baseline correction
- Standard curve creation with multiple regression models
- Qualitative analysis and cut-off determination
- Quality control with Westgard rules
- Kinetic rate calculation and slope analysis
- Full spectral analysis and peak identification
- Standard curve library for storing and recalling calibration data
Connectivity & Export
Data can be exported directly via FTP to laboratory servers or saved locally in Excel, TXT, CSV, and PDF formats. The system includes 16 GB of internal storage, accommodating more than 20,000 test records. Physical connectivity includes two USB-A ports, one USB-B port, and an Ethernet interface for network integration.
Technical Specifications
| Model | MBI Evolution UV-Vis Microplate Reader (200-1000nm) |
|---|---|
| Light Source | Xenon flash lamp (10⁹ flashes rated lifetime) |
| Wavelength Range | 200 – 1000 nm (1 nm steps) |
| Wavelength Selection | Grating monochromator |
| Wavelength Accuracy | ±2 nm |
| Wavelength Reproducibility | 0.2 nm |
| Bandwidth | <2.5 nm |
| Read-Out Range | 0 – 4.0 OD |
| Detector | Dual silicon photoelectric detectors (measurement + reference) |
| Linearity @ 450nm | R² ≥ 0.999 (0.0 – 3.0 Abs) |
| Accuracy @ 450nm | ±(1.0% + 0.003 Abs) [0 – 2.0 Abs]; ±2.0% [2.0 – 2.5 Abs] |
| Precision @ 450nm | CV < 0.5% or SD < 0.003 (Accurate mode); CV < 1.0% (Fast mode) |
| Test Speed (96-well) | 8 seconds (Fast mode) / 28 seconds (Accurate mode) |
| Plate Shaking | Linear, 3 speed options |
| Incubator | RT + 4°C to 45°C |
| Microplate Formats | 96-well and 384-well plates (with or without lids) |
| Display | 10-inch capacitive touchscreen (Android OS) |
| User Interface | Touchscreen, external keyboard/mouse compatible |
| Analysis Software | MBI ReaderIt-II (PC) / Integrated Android software |
| Storage | 16 GB (>20,000 test records) |
| Ports | 2× USB-A, 1× USB-B, 1× Ethernet |
| Automation | Not compatible with automated systems |
| Power Supply | DC 24 V, 6.67 A |
| Dimensions (W × D × H) | 300 × 500 × 260 mm |
| Weight | 15.5 kg (35 lbs) |
Frequently Asked Questions
What is the wavelength range of the MBI Evolution microplate reader?
The MBI Evolution UV-Vis Microplate Reader covers a full UV-Vis range of 200 to 1000 nm with 1 nm wavelength selection steps, using a precision grating monochromator system. This enables full spectral scanning, UV nucleic acid quantification, and visible-range colorimetric assays on a single instrument.
Is the MBI Evolution compatible with 384-well plates?
Yes. The MBI Evolution is fully compatible with both 96-well and 384-well microplates, with or without lids. The optical system is optimized for both formats, delivering accurate absorbance readings across all well densities.
What is the difference between a monochromator and filter-based microplate reader?
A monochromator-based microplate reader uses a diffraction grating to select any wavelength within a continuous range, eliminating the need to purchase or change filters. This offers greater flexibility, lower long-term operating costs, and full spectral scanning capabilities compared to filter-based readers, which are limited to pre-installed filter wavelengths.
What assays can I run on the MBI Evolution?
The MBI Evolution supports a wide range of absorbance assays including ELISA, protein quantification (BCA, Bradford, Lowry), nucleic acid detection and purity assessment (A260/A280), enzyme kinetics, cell viability (MTT, XTT, resazurin), and full spectral scanning of unknown compounds.
Does the MBI Evolution have incubation and shaking functions?
Yes. The MBI Evolution includes an incubator with a temperature range of RT+4°C to 45°C and linear shaking with three selectable speed options. These features are essential for cell-based assays, temperature-sensitive enzyme reactions, and homogeneous mixing protocols.
How fast is the MBI Evolution for 96-well plate reading?
The MBI Evolution reads a full 96-well plate in 8 seconds in Fast mode, or 28 seconds in Accurate mode. The Accurate mode provides higher precision (CV < 0.5%) for demanding quantitative assays, while Fast mode maximizes throughput for screening applications.
Can the MBI Evolution operate without a computer?
Yes. The MBI Evolution features a built-in 10-inch Android touchscreen with integrated software that supports endpoint, kinetic, and spectral scanning protocols independently. For advanced data analysis, standard curve libraries, and multi-format export, the system can also be controlled by MBI ReaderIt-II PC software.
Is the MBI Evolution suitable for Canadian research laboratories?
Yes. The MBI Evolution is distributed across Canada by Montreal Biotech Inc., with full technical support, service, and warranty coverage. The instrument operates on standard laboratory power and is backed by a Canadian-based support team for installation, training, and maintenance.
| Light Source | Xenon Lamp |
|---|---|
| Precision | @450nm = CV <0.5 % or SD<0.003 accurate model; CV <1.0 % fast mode |
| Accuracy | @450nm = ±(1.0 %+0.003Abs),(0~2.0 Abs]; ±2.0 %,(2.0~2.5Abs] |
| Wavelength Selection | Monochromator |
| Read-out Range | 0 - 4.0 OD |
| Half-Bandwidth of Filters | <2.5 nm |
| Wavelength range | 200 - 1000nm with 1 nm steps |
| Wavelength Accuracy | ± 2nm |
| Test Speed | 8 seconds with 96-well plate (fast mode); 28 seconds with 96-well plate (accurate model) |
| Incubator | RT + 4 ℃~45 ℃ |
| UI | Integrated software or PC control software |
| Analysis software | MBI ReaderIt-II |
| User Interface | Touch screen, android system, 10 inch touch screen, external keyboard mouse |
| Storage | 16G, more than 20,000 test records |
| Automated Systems | Temporarily unable compatible with automated systems |
| Detector | Two silicon photoelectric detector, one for measurement, another for reference |
| Shaking | Linear; three options for speed |
| Linearity (±) | R²≥0.999,[0.0-3.0Abs] |
| Display | 10 inch touch screen |
| Dimensions | 300×500×260 mm |
| Net Weight | 15,5 Kg (35 lbs) |
| Power Supply | DC24 V, 6.67 A |
| Microplate Formats | 96-and 384-well plates |
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