Cell Culture Insert, PET, 12 Nested Inserts + 24 Well Plate, Non-Treated, 3μm, Individually Packaged in Peelable Tyvek Film & Blister, 120/cs

Cell Culture Insert, PET, 12 Nested Inserts + 24 Well Plate, Non-Treated, 3μm, Individually Packaged in Peelable Tyvek Film & Blister, 120/cs

Cell inserts are commonly used in cell experiments, such as: co-culture experiments, chemotaxis experiments, cell migration experiments, cell invasion and drug transport experiments. Permeable supports may effectively improve the culture of polar cells, since these supports allow cells to secrete on and absorb molecules from their basal and apical surfaces to metabolize in a more natural way, as well as to stimulate the in vivo environment to the maximum extent for culturing of some special cell lines.

Polycarbonate (PC)

The membrane has excellent cell adhesion and therefore is suitable for transport studies and other applications that need to achieve optimal cell growth. Besides, PC membrane is also very for barrier analysis. Compare to other membrane materials, PC membrane has higher, more persistent and more stable trans-epthelial electrical resistance (TEER) values. PC membrane can be used to culture most types of cells without matrix coating.

Features:

• Innovative edge design for convenient sampling
• Great insertion versatility, can match with multiple brands
• PC membrane: low adsorption rate, reduces the loss of small-molecule proteins and other compounds
• PET membrane: excellent transparency, providing better optical clarity for easy observation of cell status
• The membrane materials have passed the SUP Vi-grade toxicity tests and are used for the field of medical devices
• Without cytotoxin or heat source

Applications:

Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines.

This product produce a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.

Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.

Indications for Use

• Before use, add the liquid medium into the wells of the multi-well plate and put the insert in, then add the liquid medium containing cells into the insert;
• Pre-balance: add the liquid medium into the multi-well plate and put the insert in, then put the whole thing into an incubator for at least 1 hour, or overnight;
• Check the volume of the liquid medium regularly, and replenish fresh medium, if necessary.
• Nevertheless, the cell layers may be fixed and stained directly in the insert using basic cytological methods. Note: avoid using solvents that may dissolve PC membranes.
• Fix and stain the cells in the insert directly and the membrane may be cut off with a scalpel for long-term storage.

Precautions

• Adhesion of the cells growing on the permeable support (membrane) is sensible to the starting inoculation density, so it is necessary to use different inoculation densities to ensure optimum growth.
• During the culture process, when aspirating or adding liquid through the interspace between the upper layer and the well, please be careful and go slowly to avoid membrane damage;
• Before culturing cells, incubating the permeable support in the medium may promote the adhesion and distribution of cells.
• Bubbles often occur between the lower layer of the liquid medium and the insert, once the bubbles are generated, the chemotaxis of the lower medium will be weakened or even disappears, so special care must be taken during cell inoculation. If any bubble occurs, lift the insert up to remove the bubbles and then put the insert into the plate.