Tristar 3 Multimode Reader
Tristar 3 Multimode ReaderFilter-based multimode solutionThe Tristar 3 is a user-friendly and affordable filter-based multimode plate reader that offers the high-performance analysis you expect from Berthold Technologies instruments. Equipped with ONE-4-ALL optics for uncompromised performance of all detection modes, the system is ready to perform absorbance, luminescence and fluorescence measurements. Detection TechnologiesThe Tristar 3 Multimode Microplate Reader provides you with application flexibility for today, tomorrow, and beyond to master your changing plate reading applications:
ONE-4-ALL Optics for uncompromised performanceOur patented ONE-4-ALL optics have been optimised to combine the stability and user-friendliness of a multimodal optical system with the sensitivity and versatility of dedicated optical devices. ONE-4-ALL optics work for both filter- and monochromator-based applications, so there´s never a compromise in performance in any mode. High-performance filter system - when sensitivity countsThe quick-change filter technology of the Tristar series provides you with the flexibility required to meet your application needs: up to 40 different excitation and emission filters can be easily mounted on exchangeable filter slides. The filters are characterized by high transmissions properties which can be up to 25-fold that of monochromators. Technologies like Time-Resolved Fluorescence (TRF) can be measured more efficiently with filters. Furthermore, filters with a large bandwidth are available to analyze fluorophores with wide spectra and all luminescence-based assay requiring to use filters, e.g. BRET, BRET2 and NanoBRET™ assays. Ergonomic design to support your workThe ergonomic design of the system provides full front access for all key operations (e.g. plate loading, filter change and connecting reagents). Reagent vials can be stored in an integrated front compartment, providing easy access and visibility. It contains a removable trough that can be filled with water or ice to keep all reagents cooled. Different reagent tube holders are available to ensure secure handling of valuable reagents. The system has a flat surface on its top, providing enough space to put down a laptop. Powerful crosstalk control – reliable resultsDo you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples? Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Tristar 3 does almost eliminate this problem: with a crosstalk of 10-6, you never have to worry about the position of your high-intensity samples in your plate. JET injectors: highest accuracy, speed and cell-friendlinessThe Tristar 3 can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence. Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.
Convenient Software optionsChoose between different software options according to your application needs: ICE Software
MikroWin Software
Technical Details
|
| Wavelength Selection | High quality interference filters |
|---|---|
| Measurement Technologies | Luminescence BRET, BRET² Fluorescence (top) Absorbance UV & VIS TR |
| Luminescence | 6 amol/well ATP (96 well) |
| Fluorescence | < 7 amol/well FITC (384sv) |
| Absorbance Range | Accuracy better 2% Precision better 0.6% |
| Time-Resolved Fluor. | < 35 amol/well |
| Dynamic range (linearity) | 6 orders of magnitude (photon counter) 0-3.5 OD (photodiode) |
| Crosstalk | 5 x 10-6 |
| Injection unit | up to 3 injectors, JET injection technology variable volumes: 10 – 100 μL speed 200 – 440 μL/sec accuracy better 2% (over entire range of volume) precision better 2% (over entire range of volume) injections into microplates with up to 384 wells |
| Shaking | 3 modes, variable amplitude and speed |
| Microplate Formats | 6 to 384 well, solid and strip, Dimensions 128 x 86 mm (L x W) height 14.0 – 21.0 mm (adapters necessary) Petri dishes 35 and 60 mm Eppendorf μPlate G 0.5 Standard cuvettes (with cap) |
| Interface | USB |
| PC operating system | Windows 7 (32/64 bit), Windows 10 (32/64 bit) |
| PC Requirements | Pentium like CPU (2 GHz or better / Intel Core iX recommended), 1 free USB port |
| Regulations | CE, NRTL |
| Power Supply | 100 – 240 VAC ±10% 50 / 60 Hz Class I |
| Voltage | 24 VDC ± 5% |
| Dimensions | 391 x 470 x 344 mm |
| Detector | Low-noise photomultiplier tube in dual mode, spectral range 280 - 650 nm (up to 850 nm with extended range PMT) Photo diode: spectral range 200 – 1000 nm |
| Light Source | Xenon flash lamp |
| Wavelength range | 200 - 1000nm |
