ABL1 ; ABL2 ; PDGFRB ; CRLF2 ; JAK2 gene break apart detection probe (CRLF2)

CRLF2 Gene Break Apart Probe Detection Kit (Fluorescence In Situ Hybridization Method).  - 100µL/10 Tests

CRLF2 gene encodes a member of the type I cytokine receptor family. The encoded protein is a receptor for thymic stromal lymphopoietin (TSLP). Together with the interleukin 7 receptor (IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system.

Rearrangement of this gene with immunoglobulin heavy chain gene (IGH) on chromosome 14, or with P2Y purinoceptor 8 gene (P2RY8) on the same X or Y chromosomes is associated with B-progenitor acute lymphoblastic leukemia (ALL) and Down syndrome ALL.

Product Main Components

The kit consists of CRLF2 dual-color probes

Product Intended Use
This kit performs in situ hybridization staining on the basis of conventional staining to provide physicians with auxiliary information for diagnosis. The test results are for clinical reference only and should not be used as the only basis for clinical diagnosis. Clinicians should make comprehensive judgment on the test results based on factors such as the patient’s condition, drug indications, treatment response and other laboratory test indicators.

Detection Principle
Fluorescence in situ hybridization is a technique for directly observing specific nucleic acids in cells in vitro. According to the principle of complementary base pairing, a specific probe is complementary to the target sequence in the cell. Because the probe is fluorescent, the hybridization probe and the target sequence can be clearly observed under a fluorescence microscope under the appropriate excitation light and the genetic status is observed.

Applicable Instruments
Fluorescence microscopy imaging systems, including fluorescence microscopy and filter sets suitable for DAPI (367/452), Green (495/517), and Orange (547/565).