CBFB gene break apart detection probe (CBFB)

CBFB Gene Break Apart Probe Detection Kit - 100µL/10 Tests

The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.

Product Introduction
The kit uses orange fluorescein-labeled CBFB orange probe and green fluorescein-labeled CBFB green probe to bind CBFB probe to the target detection site by in situ hybridization.

Product Main Components

The kit consists of CBFB dual color probe

Applicable Instruments
Fluorescence microscopy imaging system, including fluorescence microscope and filter set suitable for DAPI (367/452), Green (495/517) and Orange (547/565).