Human ALK Gene Fusion Detection Probe Detection Kit (ALK)

RUO - Human ALK Gene Fusion Detection Probe Detection Kit - 100µL/10 Tests

ALK amplifications, fusions and mutations have been shown to be driving events in non-small cell lung cancer. This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer.

The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumorigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).

Product Introduction
This kit uses Orange Fluorescein and Green Fluorescein labeled ALK probes to detect ALK rearrangements by in situ hybridization.

Product Main Components

This kit consists of ALK dual color probe

Applicable Instruments

1. Fluorescence microscopy imaging systems, including fluorescence microscopy and filter sets suitable for DAPI (367/452), Green (495/517), and Orange (547/565).
2. Automatic hybridization instrument: Strict temperature uniformity is required, and the temperature difference should be ≤1oC