D13S319 gene deletion detection probe (D13S319)

D13S319 Gene Deletion Probe Detection Kit (RUO) - 100µL/10 Tests

Loss of the 13q region or even the whole of chromosome 13 is very common in cases of multiple myeloma (MM). Deletions of the DS13S319 gene are common in a variety of hematological malignancies including chronic lymphocytic leukemia (CLL), acute myelocytic leukemia (AML) and MM. 

Product Main Components

The kit consists of D13S319 monocolor probes

Intended use

The reagent carries out in situ hybridization staining on the basis of routine staining to provide doctors with auxiliary information for diagnosis. The test results are only for clinical reference and should not be used as the only basis for clinical diagnosis. Clinicians should comprehensively judge the test results in combination with the patient's condition, drug indications, treatment response and other laboratory test indicators.

Detection principle

Fluorescence in situ hybridization is a technique for directly observing specific nucleic acids in cells in vitro. According to the principle of base complementary pairing, the specific probe is complementary to the target sequence in the cell. Due to the fluorescence of the probe, the gene state of the hybrid probe and the target sequence can be clearly observed under the fluorescence microscope under the appropriate excitation light.

Applicable Instruments

Fluorescence microscopy imaging systems, including fluorescence microscopy and filter sets suitable for DAPI (367/452), Green (495/517), and Orange (547/565).