IGH/CCND1 ; IGH/MAF ; IGH/MAFB ; IGH/FGFR3] gene fusion detection probe (FGFR3/IGH)
IGH/FGFR3 Gene Fusion Probe Detection Kit - 100µL/10 Tests
The FGFR proteins are involved in a wide array of pathways known to play a significant role in cancer. Activation of these receptors can lead to activation of the RAS-MAPK pathway and the PI3K-AKT pathway, among others. The mechanisms by which FGFR can be misregulated vary between cancers.
Amplification of the receptors has been observed in lung and breast cancers, coding mutations and deletions have been seen in many cancers, and more recently, FGFR fusions that lead to pathway activation have been demonstrated to have oncogenic potential across multiple cancer types. The targeted therapeutics ponatinib, dovitinib and pazopanib have seen success in treating over-active FGFR signaling, prompting use of diagnostic sequencing targeting the FGFR genes, especially in lung cancer patients.
Product Main Components
This kit consists of FGFR3/IGH dual color probe
|
Component name |
Specifications |
Quantity |
Main components |
|
FGFR3/IGH dual color probe |
100μL/Tube |
1 |
FGFR3 orange probe ; IGH green probe |
Intended use
The reagent carries out in situ hybridization staining on the basis of routine staining to provide doctors with auxiliary information for diagnosis. The test results are only for clinical reference and should not be used as the only basis for clinical diagnosis. Clinicians should comprehensively judge the test results in combination with the patient's condition, drug indications, treatment response and other laboratory test indicators.
Detection principle
Fluorescence in situ hybridization is a technique for directly observing specific nucleic acids in cells in vitro. According to the principle of base complementary pairing, the specific probe is complementary to the target sequence in the cell. Due to the fluorescence of the probe, the gene state of the hybrid probe and the target sequence can be clearly observed under the fluorescence microscope under the appropriate excitation light.
Applicable Instruments
Fluorescence microscopy imaging systems, including fluorescence microscopy and filter sets suitable for DAPI (367/452), Green (495/517), and Orange (547/565).