ABL1(9q34) gene break apart detection probe (ABL1)

ABL1(9q34) Gene Break Apart Probe Detection Kit (Fluorescence In Situ Hybridization Method).  - 100µL/10 Tests

ABL1 (ABL Proto-Oncogene 1, Non-Receptor Tyrosine Kinase) is most relevant to cancer in its role in the BCR-ABL fusion protein that has become a signature of chronic myeloid leukemia (CML). Cells harboring this fusion have shown sensitivity to imatinib, greatly improving the prognostic outlook of the disease. However, additional mutations in ABL1 have been shown to confer resistance to imatinib. In these resistance cases, second-generation tyrosine kinase inhibitors such as Dasatinib and nilotinib have exhibited some efficacy and are currently undergoing clinical trials for treating acquired resistance in CML.

Product Main Components

The kit consists of ABL1 dual-color probes

Product Intended Use
This kit performs in situ hybridization staining on the basis of conventional staining to provide physicians with auxiliary information for diagnosis. The test results are for clinical reference only and should not be used as the only basis for clinical diagnosis. Clinicians should make comprehensive judgment on the test results based on factors such as the patient’s condition, drug indications, treatment response and other laboratory test indicators.

Detection Principle
Fluorescence in situ hybridization is a technique for directly observing specific nucleic acids in cells in vitro. According to the principle of complementary base pairing, a specific probe is complementary to the target sequence in the cell. Because the probe is fluorescent, the hybridization probe and the target sequence can be clearly observed under a fluorescence microscope under the appropriate excitation light and the genetic status is observed.

Applicable Instruments
Fluorescence microscopy imaging systems, including fluorescence microscopy and filter sets suitable for DAPI (367/452), Green (495/517), and Orange (547/565).