ABL1 ; ABL2 ; PDGFRB ; CRLF2 ; JAK2 gene break apart detection probe (JAK2)

JAK2 Gene Break Apart Probe Detection Kit (Fluorescence In Situ Hybridization Method).  - 100µL/10 Tests

JAK2 is a kinase that is misregulated or mutated in a number of myeloproliferative diseases and cancers. The mutation V617F is the most clinically relevant variant, and is seen in around half of myeloproliferative disorders. The variant is a known activating mutation, and activated JAK2 is sufficient to drive myeloproliferative disorders in mouse models. V617F, while most recurrent, is not the only mechanism by which JAK2 can be activated in patients. JAK2 is now one of the first diagnostic markers tested upon diagnosis with a myeloproliferative disorder.

Product Main Components

The kit consists of JAK2 dual-color probes

Product Intended Use
This kit performs in situ hybridization staining on the basis of conventional staining to provide physicians with auxiliary information for diagnosis. The test results are for clinical reference only and should not be used as the only basis for clinical diagnosis. Clinicians should make comprehensive judgment on the test results based on factors such as the patient’s condition, drug indications, treatment response and other laboratory test indicators.

Detection Principle
Fluorescence in situ hybridization is a technique for directly observing specific nucleic acids in cells in vitro. According to the principle of complementary base pairing, a specific probe is complementary to the target sequence in the cell. Because the probe is fluorescent, the hybridization probe and the target sequence can be clearly observed under a fluorescence microscope under the appropriate excitation light and the genetic status is observed.

Applicable Instruments
Fluorescence microscopy imaging systems, including fluorescence microscopy and filter sets suitable for DAPI (367/452), Green (495/517), and Orange (547/565).