MBI RedSafe Nucleic Acid Staining Solution (0.5 ml)
MBI RedSafe Nucleic Acid Staining Solution
A safe, high-sensitivity alternative to ethidium bromide (EtBr) for DNA and RNA visualization in agarose gel electrophoresis. Non-genotoxic formulation ensures laboratory safety without compromising detection sensitivity.
Key Characteristics
- High-sensitivity detection superior to traditional EtBr staining
- Significantly reduced genotoxicity for enhanced user safety
- Dual excitation capability: UV transilluminator (standard around 280nm ) or visible light (420–510 nm)
- Emission at 520–540 nm (optimal detection with 530 or 535 nm filter)
- Compatible with both precasting and post-staining protocols
- Stable long-term storage at room temperature or 4°C
Optimal Filter Configuration
| Filter Type | MBI RedSafe Performance | Recommendation |
| 520–540 nm (Optimal) | Bright, distinct bands; maximal signal intensity | Recommended (530/535 nm) |
| 590 nm (EtBr filter) | Detectable but suboptimal brightness | Compatible with reduced sensitivity |
| No filter | Visible at reduced UV output (<40%) | Acceptable for documentation |
Spectral Properties
| Parameter | Specification |
| Excitation Wavelength | UV: 270–306 nm | Visible: 420–510 nm (480, 495 nm peaks) |
| Emission Wavelength | 535 nm (green-yellow region) |
| Optimal Detection | 530–535 nm filter |
Applications
- Visualization of DNA and RNA bands during agarose gel electrophoresis
- DNA fragment isolation for subcloning applications (minimal mutation risk)
- Routine PCR product analysis and verification
- Southern/Northern blot visualization protocols
Staining Protocols
Precasting Method: Add MBI RedSafe directly to agarose gel solution during preparation (standard protocol).
Post-Staining Method: Dilute to 1× concentration in running buffer (Example: Add 2μL of MBI RedSafe (10,000× concentrate) to 20mL of agarose solution); incubate gel for 10 minutes. Post-staining provides uniform background and enhanced small fragment detection.
Note: During extended electrophoresis runs, MBI RedSafe migrates toward the positive electrode while DNA moves toward the negative electrode, resulting in gradient background staining characteristic of intercalating dyes.
Kit Contents
| No. | Component | Quantity |
| 1 | MBI RedSafe Nucleic Acid Staining Solution | 0.5 ml × 1 vial |
| 2 | User Manual | 1 each |
Storage & Stability
| Condition | Stability | Recommendation |
| Room Temperature | ≥ 12 months | Standard storage |
| 4°C | ≥ 24 months | Extended stability |
Safety & Compliance
MBI RedSafe is designed as a safer alternative to ethidium bromide (EtBr), significantly reducing genotoxicity risks associated with traditional nucleic acid staining. Suitable for routine laboratory use without specialized hazardous waste disposal requirements (verify local regulations).