MBI EVOlution Probe (R) qPCR mix (With Rox) 20ml (5000 reactions)
For in vitro use only
Description:
MBI EVOlution 5 x Probe qPCR Mix (ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and MBI Hotstart DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.
MBI Hotstart DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Applications:
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
Mix Composition:
- MBI Hotstart DNA Polymerase
- 5 x Probe qPCR buffer
- 15 mM MgCl2 1 x PCR solution – 3 mM MgCl2
- dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP
- ROX dye
ROX Dye:
ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.
Recommended qPCR reaction mix:
Component |
Volume |
Final conc. |
MBI EVOlution 5 x Probe qPCR Mix (With ROX) |
4 µl |
1x |
Primer Forward (10 pmol/µl) |
0.4-0.8 µl |
200-400 nM |
Primer Reverse (10 pmol/µl) |
0.4-0.8 µl |
200-400 nM |
Probe |
1 µl |
100-250 nM |
DNA template |
1-5 µl |
1-50 ng/µl |
H2O PCR grade |
up to 20 µl |
|
Total |
20 µl |
|
Recommended qPCR cycles:
Cycle step |
Temp. |
Time |
Cycles |
Initial denaturation |
95ºC |
15 min |
1 |
Denaturation |
95ºC |
15-20 s |
40 |
Annealing/Elongation |
60ºC |
60 s |
IMPORTANT: To activate the polymerase, include an incubation step at 95ºC for 15 minutes at the beginning of the qPCR cycle.
Safety warnings and precautions:
This product and its components should be handled only by persons trained in laboratory techniques. It is advisable to wear suitable protective clothing, such as laboratory overalls, gloves and safety glasses. Care should be taken to avoid contact with skin or eyes. In case of contact with skin or eyes, wash immediately with water.
Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.
Shipping and Storage conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of MBI EVOlution 5 x Probe qPCR Mix (no ROX).
Amount of Reactions | 5000 Reactions |
---|---|
Mix Composition | ROX Dye |
Application | qPCR Probe |
Catalog Number | Product Name | Price | Qty |
---|---|---|---|
MBI-P250R | MBI EVOlution Probe (R) qPCR mix (With Rox) 1ml (250 reactions) |
$235.00
|
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MBI-P1000-R | MBI EVOlution Probe (R) qPCR mix (With Rox) 4ml (1000 reactions) |
$825.00
|
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