MBI Blood and Cell DNA Extraction Kit (100 assays / reactions)
Blood & Cell DNA Extraction
The magnetiQ Blood & Cell DNA Extraction Kit enables a rapid and efficient method of nucleic acid isolation from whole blood samples, as well as cell suspensions.Extraction of DNA is achieved through single-step magnetic bead-based lysis and capture of DNA from cells in blood, tissue, or cell culture samples.
Achieve highly efficient capture/elution of nucleic acids yielding purified solutions with minimal protein and salt contamination for downstream applications such as PCR and sequencing. Obtain purified and high concentrations of DNA free of cell debris, salt, lipid and protein contamination in under 15 minutes.
Performance
The magnetiQ Blood & Cell DNA Extraction Kit alleviates the need for separate lysis and binding, heating, or centrifugation steps. The purified sample can be concentrated in volumes as low as 25 μL, in contrast to column-based kits which often necessitate larger elution volumes. The magnetiQ Blood & Cell DNA Extraction Kit displays superior performance in comparison to leading commercially available column-based kits. High quantity of DNA is extracted from cell suspension using the magnetiQ Blood & Cell DNA Extraction Kit, resulting in a lower CT value for GAPDH gene.
The magnetiQ Blood & Cell DNA Extraction Kit also provides a cleaner, less contaminated eluted nucleic acid preparations from 100 μL whole blood or cell suspension samples. The kit produces very pure and high yield nucleic acid elute (ratio between 1.8-2.2) with high reproducibility.
Applications
- Next Generation Sequencing (NGS)
- PCR and qPCR gene amplification
- DNA methylation studies
- Copy number variation (CNV) studies
Features
- Amenable to high-throughput methods, including automation
- Faster and simpler magnetic collection and resuspension steps
- Reduces concern for clogging
- Eliminates organic solvent hazardous waste
- Requires only the use of a magnetic rack and standard pipettes
Specifications
The magnetiQ Blood & Cell DNA Extraction Kit contains optimized buffers for blood or cell lysis and DNA binding, washing and elution developed with Galenvs’ functionalised magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.
| Sample Type | Blood, Cells, Bacteria |
| Quantity | 100 assays (Other formats available) |
| Elution Volume | 25 µL or above |
| DNA recovered | >98% |
| Processing Mode | Automated; Manual. Compatible with 96-well plates. |
| Throughput | 100-250 samples per run |
| Binding Technology | Magnetic beads |
| Binding Capacity | Scalable |
| Components | (i) Lysis/Binding Buffer (ii) Wash Buffer #1 (iii) Wash Buffer #2 (iv) Elution Buffer (v) Proteinase K |
| Storage | Room temperature |
Video Protocol
