SHIELD - Exceptional tissue preservation (250 mL) (3 Items)
SHIELD 250ML For 8 mouse brains or samples of equivalent size - SHIELD Epoxy (SH-Ex) - SHIELD Buffer (SH-BS) - SHIELD On (SH-ON)
250 mL: Preserves 12 whole mouse brains or comparably sized samples.
- 250 mL SH-Epoxy
- 125 mL SH-Buffer
- 500 mL SH-ON
Published by Park et al. in Nature Biotechnology 2018, our SHIELD reagent (AKA GE38) safeguards endogenous fluorescence, protein antigenicity, nucleic acids, and overall tissue architecture against physical and chemical stressors. The SHIELD tissue preservation method also protects tissue during fluorescent protein imaging and antibody labeling across multiple rounds.
Compatible with:
- All PFA-fixed tissues and multiplexing
- SWITCH, MAP, SmartBatch+, and SmartLabel
Protection of tissue physicochemical properties using polyfunctional crosslinkers (Park et al., Nature Biotechnology 2018)
SHIELD is a tissue preservation technique that complements and enhances the standard PFA fixation of biological tissue. It requires a series of incubation steps over 4-6 days to preserve a tissue sample the size of a whole mouse brain. These steps allow epoxide molecules to diffuse into the tissue and crosslink, forming a strong skeleton that anchors proteins, enzymes, nucleic acids, and other important elements of tissue architecture.
This preparation step is necessary for active clearing or immunolabeling, as exposure to harsher chemicals or environments can strip away important targets in non-SHIELD-preserved tissue. The strong fixation of native proteins also allows for multiplex labelin
Quick & easy protocol
Our streamlined tissue preservation protocol takes just 4-6 days, without the variability of tissue embedding via hydrogel or paraffin. Reagents are non-toxic and can be easily disposed of after use.
| Catalog Number | Product Name | Price | Qty |
|---|---|---|---|
| SH-500 | SHIELD - Exceptional tissue preservation (500 mL) (3 Items) |
$1,025.95
|
|
| Clear+ | Active clearing : Clear+ Tissue Clearning system |
|
|
| C-PCK-250-1.52 | Passive Clearing Kit: contains preservation and clearing (250ml) |
$1,307.47
|
|
| C-PCK-500-1.52 | Passive Clearing Kit: contains preservation and clearing (500ml) |
$1,919.47
|
|
| EI-500-1.52 | EasyIndex - Optimized refractive index matching (500ml) |
$1,624.47
|
|
