Twinkle LB 970 Microplate Fluorometer (Discontinued)

This product has been discontinued.

Please see equivalent: TRISTAR 3 Multimode Reader.

For repair and maintenance please communicate with our service

department at [email protected]

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Twinkle LB 970 Microplate Fluorometer

The dedicated fluorometer is using an optimized design for a variety of measurement modes to support academic and pharmaceutical research centers.

• kinetics
• scanning
• dual ratio measurements
• top reading
• bottom reading
• temperature control

make Twinkle a valuable instrument for any researcher using fluorescence in life science.

The measurement of different plate formats, e.g. up to 384 well microplates, filters, petri dishes or Terasaki plates is possible.

The high quality design is well suited for sensitive FRET assays.

Temperature Control

The instrument can be equipped with temperature control to establish ideal conditions for all cell based applications.

Fluorescence Optics

The Twinkle LB 970 Fluorometer provides highest sensitivity due to Single Photon Counting technology and extremely low noise Photomultiplier. For excitation a Tungsten halogen lamp is used. The instrument is standard equipped with filter pairs for FITC and Umbilliferone.

The fluorescence reading can be taken from the bottom or the top. The bottom reading is instrument of choice for measurements of adherent cells, coloured medium and lids to achieve the most efficient detection.

Scanning

Especially in cell applications samples are heterogenously spread in the well. To measure the complete area, up to 100 individual measuremet points can be defined within the same well.

Shaking

3 modes each with variable speed of the integrated shaker to enhance mixing and optimise sample distribution before measurement in long term kinetics.

Kinetics

Kinetic measurements can be performed up to 7 days.

Applications

• Binding studies
• Cell proliferation
• DNA Quantification
• Drug screening
• Enzyme kinetics
• Environmental toxins
• FRET
• Immunoassays
• Phagocytosis
• Protein Concentration
• Reporter Gene Assays

Features

• Highest sensitivity due to Single Photon Counting technology and extremely low noise PMT
• Plate formats: 6, 12, 24, 48, 96 and 384 well. Tolerance for plate heights: 10 – 25 mm. Petri dishes and Terasaki plate with respective adapters.
• Plate shaking: orbital, linear and double orbital. well scanning with up to 10,000 individual reading points per well. measurement time per well user-defineable via software: between 0.1 aund 600 sec.
• Excitation unit: Tungsten halogen lamp, 75 W, spectral range 340–700 nm, typical life time >300 h. Filter slide with 5 positions (Ø 15 mm), equipped with high quality interference filters 355 nm (Umbelliferone) and 485 nm (Fluorescein).
• Detection unit: Photomultiplier tube (low noise photocathode, high cathode sensitivity, digital photon counting technology). Filter slide with 4 positions (Ø 25,4 mm), equipped with high quality interference filters 460 nm (Umbelliferone) and 535 nm (Fluorescein).
• Performance: spectral range 340 - 630 nm. detection limit for Fluorescein <1 fmol/well.

MikroWin Lite software

• Windows software for instrument control and data evaluation.
• graphical user interface with navigation bar.
• support of all typical plate formats.
• Instrument control: definition of measurement times. definition of reading area and selection of plate format.
• free positioning of well functions (samples, standards, controls) definition of sample names.
• qualitative cut-off determination with up to 3 categories.
• ratio calculation.
• Kinetics evaluation (e.g. min/max figures, Vmax, AUC, time constants).
• Scanning evaluation.
• Combination of different evaluation formula.
• Open input of user-defined formula in up to 5 matrices.
• display in multi matrix format or in list format.
• graphical display of data.
• integrated statistics functions (e.g. average, standard deviation).
• export of all raw data or calculated data in matrix or list format.
• manually or automatically executable.
• export as file (LAN connection possible) or to printer.
• OS: Windows® 98, 2000, NT, XP.
• PC system mandatory for operation.

Accessories and Options

• Temperature control: activation via software: 5 °C above RT to 40 °C. convection-type for heating of complete measurement compartment.
• Fluorescence bottom reading. Fibre optic system for excitation and transduction of emission signal. Recommended with quenching samples or measurements with lid.
• Additional excitation filter slide: 5 positions without filters.
• Additional emission filter slide 4 positions without filters.
• Tungsten halogen lamp 75 W.
• Petri dish adapter Falcon for BD Falcon Petri dishes with diameters of 35 and 60 mm.
• Petrisdish Adapter Nunc for Nunc Petri dishes with diameters of 35 and 60 mm
• Adapter for Terasaki plates
• Robot Integration Module: software patch for control and communication with software of laboratory automation systems. documentation of communication language