SmartSPIM Light Sheet Microscope
Light sheet imaging system optimized for resolution, speed, and flexibility for large samples
SmartSPIM is an advanced light sheet microscope capable of imaging large tissue samples with high speed and resolution. The system was designed from the ground up, with no limitation based on commercially available microscope bodies. The unique illumination arm of the SmartSPIM creates a flat-top illumination profile laterally, and a focused beam axially.
To achieve homogeneous axial point spread functions (PSF) across the entire FOV, the illumination optics scan the beam axially while synchronized to the camera’s detection.
Features & Highlights
- Capable of imaging a wide range of sample sizes
- High axial resolution with many different objectives
- Uniform axial resolution across the entire sample
- High speed volumetric imaging (able to image a mouse hemisphere in less than 15 minutes or a whole mouse brain in less than 25 minutes at 1.75µm x 1.75µm x 4µm voxel size)
- Ability to use custom and commercially available objectives
- Custom illumination and detection optics optimized for all current large format objectives and camera
- Automated focus compensation for RI mismatches and chromatic focal shifts
- Compatible with both aqueous and organic clearing methods
Specifications
|
Light Sheet Formation |
Dynamically axially swept light sheet |
|
Specimen Lateral Size |
2.5 cm x 2.5 cm (standard) 4 cm x 6.5 cm (extended) |
|
Illumination Optics |
Custom designed objectives, NA = 0.15, broadband chromatically corrected |
|
Detection Optics |
180 mm EFL tube lens Objectives: 1.63X, 3.6X, 15X, 22X |
|
Field of View (FOV) |
3.6X: 3650 μm 15X: 850 μm |
|
Lateral Sampling |
3.6X: 1.8 μm/pixel 15X: 0.42 μm/pixel |
|
Axial Resolution |
3.6X : NA = 0.2, PSFz = 3.2-4.0 μm 15X : NA = 0.4, PSFz = 1.4-2.2 μm |
|
Camera |
2048 x 2048 sCMOS with rolling shutter synchronized to swept lightsheet |
|
Imaging Rate |
20 FPS during volumetric acquisition |
|
Laser Lines |
Up to 6, 405 - 785 nm |
