MBI Magnetic Plasmid MINIPREP KIT (100 assays / reactions)

Plasmid Miniprep

The magnetiQ Plasmid Miniprep Kit enables a rapid and efficient method of plasmid extraction and purification without need for RNase supplement. Extraction of plasmid supercoiled DNA is achieved through single-step magnetic bead-based lysis and capture of plasmids from transformed bacteria.

Achieve highly efficient capture/elution of molecular biology grade plasmids yielding purified solutions with minimal RNA, protein and salt contamination for downstream applications such as transfection, PCR, and sequencing. The magnetiQ Plasmid Miniprep Kit enables simple and rapid plasmid extraction from cultured bacteria in 15-20 minutes using magnetic nanoparticles. No need for spin columns and multiple centrifugation steps. Isolate highly pure concentrations of plasmid DNA with negligible gDNA, protein or endotoxin carryover.

Performance

The magnetiQ Plasmid Miniprep Kit alleviates the need for centrifugation steps and separate collection tubes – in contrast to standard column-based kits. magnetiQ Plasmid Miniprep Kit allows for facile bacterial lysis, plasmid binding, and endotoxin removal only using a standard magnetic rack. The purified plasmid can be concentrated in volumes as low as 25 μL, compared to column-based kits which often necessitate larger elution volumes. The magnetiQ Plasmid Miniprep Kit performance demonstrates high yield and pure plasmid extraction for both low and high copy vectors. Sample processing is completed in less than 20 minutes, yielding high purity and efficient recovery of molecular biology grade plasmids.

Applications

• Molecular biology
• In Vitro transfection
• PCR, sequencing, cloning

Features

• Amenable to high-throughput methods, including automation
• Faster and simpler magnetic collection and resuspension steps
• Reduces concern for clogging
• Eliminates RNase treatment
• Eliminates centrifugation steps with bacterial lysis and removal of cell debris, proteins, and denatured gDNA

Specifications

The magnetiQ Plasmid Miniprep Kit contains optimized buffers for molecular biology applications developed with Galenvs’ functionalised magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.