EVE™ HT A26 - 96 Samples in 10 Minutes
Catalog Number:
EVE-HT-A26
Availability:
In stock
EVE™ HT A26 Fully Automated Cell Counter
SKU: EVE-HT-A26
The EVE™ HT A26 is a fully automated cell counting system engineered to streamline sample preparation and analysis from start to finish. By integrating hands-free pipetting, aliquoting, diluting, staining, and counting within a single benchtop platform, it delivers high-throughput performance while reducing workflow variability and ensuring reproducible results across users and runs.
Key Highlights: 96 samples in 10 minutes • Fully automated workflow — pipetting, aliquoting, diluting, staining, counting • Dual fluorescence (AO / DAPI) + brightfield channels • Compatible with AO/DAPI, Trypan Blue, and Erythrosin B staining • 8-channel automated pipette head • 20 µL per count (Direct mode) / ≥ 40 µL per count (Full mode) • Auto aliquoting & dilution • Windows 11 LTSC • Optional 21 CFR Part 11 compliance
Core Features
Complete Hands-Free Automation After loading consumables and samples on the deck, the system automatically performs the entire cell counting workflow — including aliquoting, diluting, mixing, loading, and counting — with no intermediate user intervention required.
High-Throughput 96-Sample Processing Processes up to 96 samples in just 10 minutes. The integrated 8-channel pipette head and dual counting plate holders accommodate two 48-channel counting plates simultaneously for maximum throughput.
Triple-Mode Detection Dual fluorescence channels (AO and DAPI) combined with brightfield imaging support multiple staining protocols. AO/DAPI mixed solution excludes debris and RBCs for accurate nucleated cell counts, while Trypan Blue and Erythrosin B compatibility extends application flexibility.
Consistent Results Across Users Automated liquid handling and standardized staining protocols eliminate user-to-user variability. The system delivers reproducible performance independent of operator experience, critical for regulated and high-volume environments.
Automated Workflow
The EVE™ HT A26 significantly reduces hands-on working time by automating both sample preparation and counting:
- Sample Plating — Load samples into the 96-well sample plate
- Deck Setup — Place counting plates, staining reservoirs, and tips on the deck
- Sample Staining — Automated pipetting of staining solutions (AO/DAPI, Trypan Blue, or Erythrosin B)
- Sample Loading — Automated transfer of stained samples to counting plates
- Cell Counting — Automated image acquisition and analysis
- Results Review — Software displays total cells, viability, and cell size data
Hardware Features
- 8-Channel Pipette Head — Precisely handles reagents and cell samples with disposable tips for reliable performance
- Reservoir Holder — Securely holds reagent reservoirs for staining and assay preparation
- Counting Plate Holder — Accommodates up to two counting plates (48 channels each) for high-throughput analysis
- Well Plate Holder — Holds both a 96-well sample plate and a mixing plate simultaneously
- Lift-Up Door — Auto-darkening interlocked door ensures user safety and prevents external light interference during counting
- Pipette Tip Holder — Compatible with 200 µL pipette tips for seamless automated liquid handling
Accurate & Consistent Counting Results
Dual fluorescence and brightfield channels support AO/DAPI, Trypan Blue, and Erythrosin B staining, while built-in auto aliquoting and dilution further streamline operation. This standardized workflow delivers consistent performance regardless of user experience. The fluorescence-based method distinguishes debris and counts only nucleated cells, ensuring accurate results even in complex samples such as PBMCs and primary cell preparations.
Technical Specifications
| Specification | Details |
|---|---|
| SKU / Cat. No. | EVE-HT-A26 |
| Analysis Time | 96 samples in 10 minutes |
| Sample Capacity | 96 samples per run |
| Sample Volume (Direct Mode) | 20 µL per count |
| Sample Volume (Full Mode) | ≥ 40 µL per count |
| Pipette Head | 8-channel automated pipette head |
| Channels | Dual fluorescence (AO & DAPI) + Brightfield |
| Staining Solutions | AO/DAPI mixed solution, Trypan Blue stain, Erythrosin B stain |
| Measuring Range (Detectable) | 1 × 10⁴ – 2 × 10⁷ cells/mL |
| Measuring Range (Optimal) | 1 × 10⁵ – 1 × 10⁷ cells/mL |
| Cell Size Range (Detectable) | 1 – 85 µm |
| Cell Size Range (Optimal) | 5 – 80 µm |
| Operating System | Windows 11 LTSC |
| Power | 100–240 VAC, 50/60 Hz, 400 W |
| Dimensions (W × D × H) | 700 × 690 × 856 mm |
| Weight | 147 kg |
| 21 CFR Part 11 | Available (optional compliance module) |
Applications
- High-throughput cell line screening and bioprocess development
- Cell therapy manufacturing — isolation, expansion, harvest, formulation
- PBMC and whole blood cell analysis with RBC exclusion
- Stem cell and primary cell culture monitoring
- Regulated GMP environments with optional 21 CFR Part 11 compliance
- Automated sample dilution and aliquoting for assay preparation
EVE™ HT A26 counting plates, staining reagents, and pipette tips are required for operation and are sold separately. Specifications subject to change without notice.
| Cell Size Range | 1 ~ 85 μm |
|---|---|
| Loading sample volume | 30µL per count |
| Dimensions | 700 x 690 x 856 mm |
| Net Weight | 147 kg |
| Measuring range | Detectable range: 1 x 10E4 to 2 x 10E7 cells/mL Optimal range: 1 x 10E5 to 1 x 10E7 cells/mL |
| Laser | Dual fluorescence; Bright field |
| Analysis time | 96 samples in 10 minutes |
| Dyes | Trypan blue or Erythrosin B, AO/DAPI |
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