ADAM-MC2 Cell Counter
Catalog Number:
ADAM-MC II
Availability:
In stock
ADAM-MC2 Automated Fluorescence Cell Counter
SKU: ADAM-MC II
The ADAM-MC2 is a new standard in automated fluorescence cell counting. Utilizing sensitive fluorescence dye staining (PI or AO/PI), LED optics, and CMOS detection technologies, it delivers accurate and reliable cell analysis in under 25 seconds. The system measures total cells, viable cells, non-viable cells, and viability percentage through automated image analysis — combined with a disposable microfluidic chip for simple, easy, and cost-effective operation.
Key Highlights: < 25 sec / test (4-channel) • PI or AO/PI fluorescence staining • Automated image analysis with CMOS detection • Auto-focusing • Accurate count of aggregated and irregular-shaped cells • Debris exclusion • R² = 0.9976 correlation with flow cytometry • 3-step operation • Cell therapy QC ready
Core Features
Fast — Less Than 25 Seconds
Complete total cell count, viable cell count, non-viable cell count, and viability percentage in under 25 seconds per test using the 4-channel chip. The 2-channel configuration completes in under 50 seconds.
Easy — 3-Step Operation
Prepare the sample, load the disposable microfluidic chip, and get the result. No manual counting, no hemocytometer, no expert training required. Combined with disposable AccuChip kits, operation is extremely simple and cost-effective.
Accurate — Nucleus-Based Detection
Fluorescence dyes (PI or AO/PI) intercalate DNA to stain the nucleus of target cells. Sensitive CMOS detection and precise automatic stage movement ensure accurate and reliable results with R² = 0.9976 correlation to flow cytometry.
Aggregated & Irregular-Shaped Cells
Proprietary image analysis software counts aggregated cells individually based on cell size and shape, and excludes debris from results. Accurate counting even for challenging clumped cell lines such as HepG2 and HeLa.
Principle of Viability Measurement
The ADAM-MC2 uses two fluorescence staining methods:
- PI (Propidium Iodide) Staining: PI is a membrane-impermeant dye that stains the nuclei of non-viable cells. Viable cells exclude PI. Total cell count is determined by PI-stained nuclei after lysis solution treatment.
- AO/PI (Acridine Orange / Propidium Iodide) Staining: AO stains all nucleated cells (viable and non-viable), while PI stains only non-viable cells. This dual-stain approach provides total cell count and viability in a single measurement.
After staining, the ADAM-MC2 automatically takes fluorescent images and processes them through integrated image analysis software. Two types of disposable chips are available: 2-channel and 4-channel configurations.
Cell Therapy Applications
The ADAM-MC2 serves as a QC device to monitor cell numbers and viability throughout the cell therapy manufacturing process:
- Source tissue → Cell isolation → Cell expansion → Cell harvest → Infusion to patients
Compatible cell types include:
- Stem cells
- CAR-T cells
- CAR-NK cells
- Adipose-derived stem cells
- Whole blood cells
- PBMCs (peripheral blood mononuclear cells)
- Aggregated and irregular-shaped cell lines
Accuracy & Reproducibility
Independent validation confirms high correlation and repeatability:
- Flow cytometry correlation: R² = 0.9976 (SH-SY5Y cells)
- Repeatability across concentrations: Low, medium, and high cell concentrations show consistent CV values under 4%
- Viability comparison: SH-SY5Y, Jurkat, and HeLa cells treated with H₂O₂ show comparable viability results between ADAM-MC2, flow cytometry, and manual counting
Technical Specifications
| Specification | Details |
|---|---|
| SKU / Cat. No. | ADAM-MC2 |
| Analysis Time | < 25 sec / test (4-channel) < 50 sec / test (2-channel) |
| Loading Volume | 13 µL / test (4-channel) 23 µL / test (2-channel) |
| Measuring Volume | 3.4 µL / test (4-channel) 8.6 µL / test (2-channel) |
| Measurement Range (PI) | 5 × 10⁴ – 4 × 10⁶ cells/mL |
| Measurement Range (AO/PI) | 5 × 10⁴ – 2 × 10⁷ cells/mL |
| Staining Method | Propidium Iodide (PI) or Acridine Orange / PI (AO/PI) |
| Detection Technology | CMOS detection with LED optics |
| Light Source | 4W Green LED |
| Focusing | Auto-focusing |
| Image Analysis | Automated integrated software |
| Chip Type | Disposable microfluidic chip (2-channel or 4-channel) |
| Dimensions (L × W × H) | 277 × 276 × 270 mm |
| Net Weight | 7.0 kg |
Related Accessories
- AccuChip Kit (4-channel): Cat. No. AD4K-200 — Total cell counting by PI with lysis solution
Note: AccuChip kits and staining reagents are required for operation and are sold separately. The ADAM-MC2 uses disposable microfluidic chips (2-channel or 4-channel configurations) matched to your staining protocol.
| Analysis time | < 25 sec/test (4 ch) |
|---|---|
| Loading sample volume | 13µL/test (4 ch) |
| Measuring volume | 3.4 µL/test (4ch) |
| Measuring range | 5 X 10E4 to 4 X 10E6 cells/mL(PI) 5 X 10E4 - 2 X 10E7 cells/mL (AO/PI) |
| Focusing | Auto-focusing |
| Net Weight | 7.0 kg |
| Dimensions | (L x W x H) 277 x 276 x 270 mm |
| Laser | 4W Green LED |
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