EVE™ HT FL High-Throughput Automated Fluorescence Cell Counter
EVE™ HT FL — High-Throughput Fluorescence Cell Counter
The EVE™ HT FL is a high-throughput fluorescence cell counter engineered for biopharmaceutical process development and cell therapy manufacturing QC. Combining brightfield imaging with dual-fluorescence channels (AO/DAPI), it processes 48 samples in a single run using a disposable 48-channel plate compatible with multi-channel pipettes. The system delivers accurate total cell counts, viability percentages, and cell size distributions for cell lines, primary cells, stem cells, and PBMCs — with minimal sample consumption and optional 21 CFR Part 11 compliance for regulated environments.
Core Features
High-Throughput 48-Sample Processing
The disposable 48-channel counting plate is designed for multi-channel pipette compatibility, enabling efficient loading and mixing without the delays of manual hemocytometer counting. The entire 48-sample run completes in under 4 minutes, making the EVE™ HT FL ideal for:
- Cell line screening and media optimization campaigns
- Bioprocess development and scale-up monitoring
- QC release testing in cell therapy manufacturing
- High-frequency viability monitoring across multiple conditions
Complex Sample Accuracy — PBMCs, Stem Cells, CAR-T
Traditional brightfield counting struggles with contaminated or complex samples containing red blood cells, platelets, and cellular debris. The EVE™ HT FL's dual-fluorescence approach identifies nucleated cells only, delivering accurate counts where Trypan Blue methods fail. This makes it particularly effective for:
- PBMC preparations from whole blood or apheresis products
- Stem cell and iPSC culture monitoring
- CAR-T cell manufacturing and potency assessment
- Primary cell cultures with high debris content
Performance Validation
- CHO cells: R² = 0.996
- PBMCs: R² = 0.9956
- CHO: R² = 0.9991
- Jurkat: R² = 0.9995
Software & Data Management
The intuitive software interface displays total cell count, live/dead distribution, viability percentage, and cell size histograms in real time. Export capabilities include:
- One-click PDF test reports for regulatory submissions
- CSV raw data export for downstream analysis
- JPG image export for presentations and documentation
- Real-time bar graphs and scatter plots during acquisition
- Windows 10 / Windows 11 compatible
21 CFR Part 11 / GMP Compliance (Optional)
The optional compliance module safeguards all records and data in accordance with FDA requirements for electronic records and signatures. Features include:
- User management and role-based access control
- Electronic records and electronic signatures
- Complete audit trail logging every action with date, time, and user details
- Data modification restricted to authorized users only
Technical Specifications
| Specification | Details |
|---|---|
| SKU / Cat. No. | EVE-HT-FL |
| Product Name | EVE™ HT FL — High-Throughput Fluorescence Cell Counter |
| Channels | Brightfield + Dual Fluorescence (AO / DAPI) |
| Sample Capacity | 48 samples per run |
| Analysis Time | Total < 4 min (48 samples) / Standard < 4 min / Average < 1.7 min per channel |
| Loading Sample Volume | 20 µL per channel |
| Measuring Volume | 0.4 µL per test |
| Fields of View | 15 FOV per channel |
| Concentration Range (Detectable) | 1 × 10⁴ – 4 × 10⁷ cells/mL |
| Concentration Range (Optimal) | 1 × 10⁵ – 1 × 10⁷ cells/mL |
| Cell Size Range (Detectable) | 5 – 60 µm |
| Cell Size Range (Optimal) | 8 – 40 µm |
| Staining Dye | Acridine Orange (AO) / DAPI |
| Plate Type | Disposable 48-channel counting plate, multi-channel pipette compatible |
| 21 CFR Part 11 | Optional compliance module available |
| Operating System | Windows 10 / Windows 11 compatible |
| Dimensions (W × D × H) | 550 × 437 × 558 mm / 21.6 × 17.2 × 22.0 in |
| Weight | 50 kg (110 lb) |
Applications
- High-throughput cell line screening and bioprocess development
- PBMC counting and viability assessment in blood-derived products
- Stem cell and iPSC culture monitoring and expansion QC
- CAR-T cell manufacturing, potency assessment, and release testing
- Primary cell analysis in high-debris or RBC-contaminated samples
- Regulated GMP manufacturing with optional 21 CFR Part 11 compliance
- Media optimization and cell growth curve studies
EVE™ HT FL counting plates and staining reagents sold separately. Specifications subject to change without notice.
| Cell Size Range | Detectable size: 5 ~ 85 μm Optimal size: 5 ~ 80 μm |
|---|---|
| Loading sample volume | 20μL / channel |
| Dimensions | 586 (W) x 461 (D) x 458 (H) mm |
| Net Weight | 58 kg |
| Measuring range | Detectable range: 1 x 10E4 to 2 x 10E7 cells/mL Optimal range: 1 x 10E5 to 1 x 10E7 cells/mL |
| Laser | Bright field, UV, Blue LED |
| Analysis time | 3 - 18 minutes / 48 tests |
| Dyes | Trypan blue, AO/DAPI |
| Catalog Number | Product Name | Price | Qty |
|---|---|---|---|
| EVS-100 | EVE - Cell Counting Slides, 100 slides (200 counts with 2 ea x 1.5ml of trypan blue 0.4 % ) |
$330.00
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| EVS-2500 | EVE - Cell Counting Slides, 2500 slides (5000 counts with 25 ea x 1.5ml of trypan blue 0.4 % ) |
$6,601.00
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| EVS-500 | EVE - Cell Counting Slides, 500 slides (1000 counts with 10 ea x 1.5ml of trypan blue 0.4 % ) |
$1,600.00
|
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| EVS-050 | EVE - Cell Counting Slides, 50 slides. (100 counts with 1 ea x 1.5ml of trypan blue 0.4 % ) |
$178.00
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