EVE™ HT FL High-Throughput Automated Fluorescence Cell Counter
EVE™ HT FL
High-Throughput Fluorescence Cell Counter for Biopharmaceutical Process Development
48
Samples per Run
3 min
Total Analysis Time
20 µL
Sample Volume
15 FOV
Fields of View
Engineered for Speed, Precision & Compliance
The EVE™ HT FL combines brightfield imaging with dual-fluorescence channels (AO/DAPI) to deliver accurate, high-throughput cell counting for cell lines, primary cells, stem cells and PBMCs.
Unmatched Throughput
Process up to 48 samples in a single 3-minute run using a disposable 48-channel plate compatible with multi-channel pipettes.
Dual Fluorescence Staining
AO stains all nucleated cells; DAPI selectively stains non-viable cells. Effectively excludes RBCs, platelets and debris from complex samples like PBMCs.
15 Fields of View
Increased FOV reduces statistical errors from uneven cell distribution, enhancing accuracy and repeatability across users and labs.
21 CFR Part 11 / GMP Ready
Optional compliance module with electronic records, audit trails, electronic signatures and role-based user management for cGMP facilities.
Minimal Sample Consumption
Only 20 µL per channel preserves precious primary cells, stem cells and biopharma samples while maintaining precise measurement.
Flow Cytometer Correlation
R² > 0.995 correlation with flow cytometry for CHO and PBMC cells. Excellent linearity (R² > 0.999) and low instrument-to-instrument variability.
48 Samples in One Run
The 48-well disposable counting plate is designed for multi-channel pipette compatibility, letting you load and mix samples efficiently without manual hemocytometer delays.
- Disposable 48-channel plate
- Multi-pipette compatible loading
- Less than 3 minutes total run time
- Ideal for QC and process development
Count PBMCs Only — Exclude RBCs & Debris
Traditional brightfield counting struggles with contaminated samples. EVE™ HT FL's dual-fluorescence approach identifies nucleated cells only, delivering accurate counts where trypan blue fails.
- Excludes non-nucleated RBCs and platelets
- Eliminates debris interference
- Superior accuracy vs. Trypan Blue
- Perfect for CAR-T and stem cell manufacturing
Outpace the Competition
EVE™ HT FL processes 48 samples in 3 minutes with only 20 µL per channel — far surpassing manual counting and competing automated systems.
Proven Accuracy You Can Trust
Independent validation shows exceptional correlation with flow cytometry and outstanding linearity across dilution series.
High Correlation with Flow Cytometry
CHO cells: R² = 0.996 | PBMCs: R² = 0.9956
Excellent Linearity (ISO Standard)
CHO: R² = 0.9991 | Jurkat: R² = 0.9995
Visualized Data at a Glance
The intuitive software displays total cell count, live/dead distribution, viability percentage and cell size histograms in real time. Export results as PDF reports, CSV raw data or JPG images for presentations and regulatory submissions.
- Real-time bar graphs and scatter plots
- One-click PDF test reports
- CSV and JPG batch export
- Windows 10 / 11 compatible
Specifications
| Parameter | Value |
|---|---|
| Product Name | EVE™ HT FL — High-Throughput Fluorescence Cell Counter |
| Channels | Brightfield + Dual Fluorescence (AO / DAPI) |
| Concentration Range | Detectable: 1 × 10⁴ – 4 × 10⁷ cells/mL | Optimal: 1 × 10⁵ – 1 × 10⁷ cells/mL |
| Cell Size | Detectable: 5 – 60 µm | Optimal: 8 – 40 µm |
| Loading Volume | 20 µL / channel |
| Analysis Time | Total < 4 min (48 samples) | Standard < 4 min | Average < 1.7 min/channel |
| Staining Dye | AO (Acridine Orange) / DAPI |
| 21 CFR Part 11 | Available (optional GMP-compliance module) |
| Operating System | Windows 10 / Windows 11 compatible |
| Dimensions (W × D × H) | 550 × 437 × 558 mm / 21.6 × 17.2 × 22.0 in |
| Weight | 50 kg (110 lb) |
21 CFR Part 11 / GMP Ready
EVE™ HT FL is ready for regulated environments with an optional compliance module designed for cGMP facilities.
Ready to Accelerate Your Cell Counting Workflow?
Get a tailored quote for the EVE™ HT FL, counting plates and consumables.
| Cell Size Range | Detectable size: 5 ~ 85 μm Optimal size: 5 ~ 80 μm |
|---|---|
| Loading sample volume | 20μL / channel |
| Dimensions | 586 (W) x 461 (D) x 458 (H) mm |
| Net Weight | 58 kg |
| Measuring range | Detectable range: 1 x 10E4 to 2 x 10E7 cells/mL Optimal range: 1 x 10E5 to 1 x 10E7 cells/mL |
| Laser | Bright field, UV, Blue LED |
| Analysis time | 3 - 18 minutes / 48 tests |
| Dyes | Trypan blue, AO/DAPI |
| Catalog Number | Product Name | Price | Qty |
|---|---|---|---|
| EVS-100 | EVE - Cell Counting Slides, 100 slides (200 counts with 2 ea x 1.5ml of trypan blue 0.4 % ) |
Special Price
$285.00
Regular Price
$330.00
|
|
| EVS-2500 | EVE - Cell Counting Slides, 2500 slides (5000 counts with 25 ea x 1.5ml of trypan blue 0.4 % ) |
Special Price
$5,995.00
Regular Price
$6,601.00
|
|
| EVS-500 | EVE - Cell Counting Slides, 500 slides (1000 counts with 10 ea x 1.5ml of trypan blue 0.4 % ) |
Special Price
$1,450.00
Regular Price
$1,600.00
|
|
| EVS-050 | EVE - Cell Counting Slides, 50 slides. (100 counts with 1 ea x 1.5ml of trypan blue 0.4 % ) |
Special Price
$160.00
Regular Price
$178.00
|
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