ADAM MC Plus Fluorescence Cell Counter
ADAM MC Plus Fluorescence Cell Counter
The ADAM MC Plus sets a new standard in highly accurate automated fluorescence cell counting. Equipped with brightfield and two fluorescence channels (AO/DAPI), it utilizes sensitive fluorescence dye staining, LED optics, and CMOS detection technologies to deliver accurate and reliable cell analysis. The system measures total cells, viable cells, non-viable cells, cell viability percentage, cell size distribution, and cell aggregation ratio — all combined with a disposable microfluidic chip for extremely simple, easy, and cost-effective operation.
Core Features
One-Step AO/DAPI Fluorescent Staining
The ADAM MC Plus uses a single mixed solution of Acridine Orange (AO) and DAPI (4′,6-diamidino-2-phenylindole) to stain total and dead cells respectively. Because AO and DAPI stain nucleated cells only, the system effectively excludes interferences such as debris and red blood cells in samples with complex backgrounds — delivering accurate cell counts and viability analysis where brightfield methods fail.
Large Measuring Volume & Expanded Field of Views
The ADAM MC Plus measures a larger volume of sample and captures more image frames per channel than competing products. By detecting multiple images of the sample through precise moving stage operation, the integrated image analysis software processes 13 captured images per channel to provide superior repeatability and accuracy. Cell counting accuracy depends on counting volume — the ADAM MC Plus's expanded measurement capability ensures more statistically significant results.
Technical Specifications
| Specification | Details |
|---|---|
| SKU / Cat. No. | ADAM-MC Plus |
| Channels | Bright field / 2 fluorescence channels (AO & DAPI) |
| Objective Lens | NF22-4X (NA 1.5) |
| Focus | Auto-focusing |
| Analysis Time | < 40 sec / test (4-channel) |
| Loading Sample Volume | 15 µL |
| Measuring Volume | 3.2 µL / 1 channel |
| Images per Channel | 13 captured images |
| Tests per Slide | 4 tests available with 1 slide |
| Measuring Range (Detectable) | 5 × 10⁴ – 2 × 10⁷ cells/mL |
| Measuring Range (Optimal) | 4 × 10⁵ – 1 × 10⁷ cells/mL |
| Staining Method | Acridine Orange (AO) / DAPI solution (single mixed solution) |
| Detection Technology | CMOS detection with LED optics |
| Result Analysis | Cell viability (%), cell size, cell aggregation ratio |
| Dimensions (W × D × H) | 277 × 276 × 270 mm |
| Net Weight | 7.0 kg |
Applications
The ADAM MC Plus is applicable to various cell types requiring accurate monitoring during research and development:
- PBMCs — Clinical immunology research
- Stem cells — Regenerative medicine development
- Primary cells — Basic research and isolation validation
- Cell lines — Routine culture monitoring and assay development
- Splenocytes — Vaccine development studies
Accuracy & Reproducibility
Independent validation confirms high correlation with reference methods:
- Flow cytometry correlation (PBMCs): R² = 0.993 (y = 0.9958x)
- Repeatability: Low CV values across low, medium, and high cell concentrations
- Large measuring volume advantage: Superior accuracy compared to competitors due to expanded counting volume and multiple image frames
| Analysis time | Less than 40 sec / test (4ch) |
|---|---|
| Loading sample volume | 15μL |
| Measuring volume | 3.2 µL/1 Channel |
| Measuring range | 5 X 10E4 to 2 X 10E7 cells/mL(Detectable) 4 X 10E5 to 1X 10E7 cells/mL(Optimal) |
| Focusing | Auto-focusing |
| Net Weight | 7.0 kg |
| Objective Lens | NF22-4X (NA1.5) |
| Channels | Bright field / 2ch (AO & DAPI) |
| Dyes | Acridine orange / DAPI solution |
| Catalog Number | Product Name | Price | Qty |
|---|---|---|---|
| AD4K-200 | Accuchip 4x Kit (max 400 tests) with Accustain Solution |
$1,034.00
|
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| DHC-N01-50 | c-chip disposable hematocymeter (Neubauer) (50 Slides / 100 Tests / 100um) |
$325.00
|
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